Relationship between the occurrence of cysteine in proteins and the complexity of organisms
Relationship between the occurrence of cysteine in proteins and the complexity of organisms
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DOI：
10.1093/oxfordjournals.molbev.a026406
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发表时间：
2000-08-01
影响因子：
10.7
通讯作者：
Csutora, P
中科院分区：
文献类型：
--
作者：
Miseta, A;Csutora, P
在各种物种的蛋白质中研究了半胱氨酸残基的发生和相对位置。考虑到由两个密码子编码的氨基酸的随机数学发生（3.28％），在所有研究的生物体中，半胱氨酸的代表性不足。半胱氨酸的表示似乎与生物体的复杂性成正相关，哺乳动物的2.26％在2.26％和0.5％的大细条阶顺序中。该观察结果以及通过比较各种核糖体蛋白的半胱氨酸含量获得的结果，表明进化利用了增加半胱氨酸残基的使用。在所有研究的生物中，除植物外，经常发现两个半胱氨酸分开的两个氨基酸残基（c-（x）（2）-c基序）。已知这种基序存在于多种金属结合蛋白和氧化还原酶中。值得注意的是，在ARCEA中的C-（x）（2）-c基序中发现了超过21％的半胱氨酸。该观察结果可能表明半胱氨酸首先出现在古代金属结合蛋白中，并在后来被引入其他蛋白质。
The occurrence and relative positions of cysteine residues were investigated in proteins of various species. Considering random mathematical occurrence for an amino acid coded by two codons (3.28%), cysteine is underrepresented in all organisms investigated. Representation of cysteine appears to correlate positively with the complexity of the organism, ranging between 2.26% in mammals and 0.5% in some members of the Archeabacteria order. This observation, together with the results obtained from comparison of cysteine content of various ribosomal proteins, indicates that evolution takes advantage of increased use of cysteine residues. In all organisms studied except plants, two cysteines are frequently found two amino acid residues apart (C-(X)(2)-C motif). Such a motif is known to be present in a variety of metal-binding proteins and oxidoreductases. Remarkably, more than 21% of all of cysteines were found within the C-(X)(2)-C motifs in Archea. This observation may indicate that cysteine appeared in ancient metal-binding proteins first and was introduced into other proteins later.