Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma
Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma
复制标题
DOI：
10.1007/s00428-010-0930-8
复制
发表时间：
2010-07-01
期刊：
影响因子：
3.5
通讯作者：
Noguchi, Masayuki
中科院分区：
文献类型：
--
作者：
Kobayashi, Hiromi;Minami, Yuko;Noguchi, Masayuki
GA 733基因家族由GA 733 - 1（TR0P2）和GA 733 - 2（Ep-CAM）组成，其表达已在各种癌中被检测，并且据报道与预后显著相关。本研究旨在探讨GA 733基因家族成员在肺腺癌中的表达及其与预后的关系。使用Noguchi等人设计的小尺寸肺腺癌分类法（Cancer 75：2844 - 2852，1995）对130个直径小于2cm的小尺寸肺腺癌石蜡包埋样本进行分类，并使用抗Ep-CAM的鼠单克隆抗体和抗TROP2的山羊多克隆抗体进行免疫组织化学检查。采用Kaplan-Meier法计算患者生存率。Ep-CAM和TROP2在许多小肺腺癌中表达相似。Ep-CAM的表达与有利的结果显著相关（p = 0.0185），而TR0P2倾向于在具有不利结果的病例中表达（p = 0.0564），并且与非上皮型腺癌的不利结果显著相关（p = 0.0125）。多因素分析显示，TROP2过表达和淋巴结转移是独立的预后指标。虽然这两种GA733蛋白具有结构相似性，但它们在小尺寸腺癌中似乎具有相反的生物学意义。由于在更低分化的肿瘤中检测到TROP2的表达，因此该蛋白可能具有致癌活性。
The GA733 gene family is composed of GA733-1 (TROP2) and GA733-2 (Ep-CAM), whose expression has been examined in various carcinomas and reported to be significantly associated with prognosis. The aim of this study was to investigate the expression of GA733 gene family members and to compare their prognostic significance in pulmonary adenocarcinoma. One hundred thirty paraffin-embedded specimens of small-sized pulmonary adenocarcinoma, less than 2 cm in diameter, were categorized using the classification of small-sized pulmonary adenocarcinoma devised by Noguchi et al. (Cancer 75:2844-2852, 1995) and examined immunohistochemically using a murine monoclonal antibody against Ep-CAM and a goat polyclonal antibody against TROP2. The patient survival rate was calculated using the Kaplan-Meier method. Ep-CAM and TROP2 were similarly expressed in many small-sized pulmonary adenocarcinomas. The expression of Ep-CAM was significantly related to a favorable outcome (p = 0.0185), whereas TROP2 tended to be expressed in cases with an unfavorable outcome (p = 0.0564), and was significantly associated with an unfavorable outcome in nonlepidic-type adenocarcinomas (p = 0.0125). Multivariate analysis showed that TROP2 overexpression and lymph node metastasis were independent prognostic markers. Although the two GA733 proteins share structural similarity, they appear to have opposite biological significances in small-sized adenocarcinomas. As the expression of TROP2 was detected in more poorly differentiated tumors, the protein may have oncogenic activity.