GLIPR1 and SPARC expression profile reveals a signature associated with prostate Cancer Brain metastasis
GLIPR1 and SPARC expression profile reveals a signature associated with prostate Cancer Brain metastasis
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DOI：
10.1016/j.mce.2021.111230
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发表时间：
2021-03-16
影响因子：
4.1
通讯作者：
Nasciutti, Luiz Eurico
中科院分区：
文献类型：
--
作者：
de Oliveira-Barros, Eliane Gouvea;Branco, Luiza Castello;Nasciutti, Luiz Eurico
尽管在致死性前列腺癌的治疗方面取得了进展，但前列腺癌脑转移的发病率正在增加。在这个意义上，我们分析了分子概况，以及前列腺肿瘤细胞和人类星形胶质细胞之间的相互作用所涉及的功能后果。我们观察到，DU 145细胞，而不是LNCaP细胞或RWPE-1细胞，表现出更明显的恶性和侵袭性表型沿着它们与星形胶质细胞的相互作用。此外，全局基因表达分析揭示了在我们的共培养模型中不同表达的几个基因，其中GLIPR 1和GIPR 2的过表达可能代表与前列腺恶性细胞侵袭中枢神经系统相关的分子特征。此外，这些结果通过免疫组织化学和计算机分析得到证实。因此，我们推测，这里提出的数据可能会增加知识的分子机制与前列腺恶性细胞的CNS的入侵。
Despite advances in treatment of lethal prostate cancer, the incidence of prostate cancer brain metastases is increasing. In this sense, we analyzed the molecular profile, as well as the functional consequences involved in the reciprocal interactions between prostate tumor cells and human astrocytes. We observed that the DU145 cells, but not the LNCaP cells or the RWPE-1 cells, exhibited more pronounced, malignant and invasive phenotypes along their interactions with astrocytes. Moreover, global gene expression analysis revealed several genes that were differently expressed in our co-culture models with the overexpression of GLIPR1 and SPARC potentially representing a molecular signature associated with the invasion of central nervous system by prostate malignant cells. Further, these results were corroborated by immunohistochemistry and in silico analysis. Thus, we conjecture that the data here presented may increase the knowledge about the molecular mechanisms associated with the invasion of CNS by prostate malignant cells.