Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia.
Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia.
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DOI：
10.1007/978-1-59745-523-7_20
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发表时间：
2009
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表面等离子体共振是一种用于检测分子相互作用的光学技术。移动的分子（分析物）与固定在薄金属膜上的分子（配体）的结合改变了膜的折射率。在偏振光撞击在膜上之后反射的光的消光角被改变，作为检测器位置的变化来监测反射强度的下降（表面等离子体共振现象）。由于该方法严格检测质量，因此不需要标记相互作用的组分，从而消除了其分子性质的可能变化。我们利用表面等离子体共振技术研究了毛细胞蛋白质之间的相互作用。
Surface plasmon resonance is an optical technique utilized for detecting molecular interactions. Binding of a mobile molecule (analyte) to a molecule immobilized on a thin metal film (ligand) changes the refractive index of the film. The angle of extinction of light, reflected after polarized light impinges upon the film, is altered, monitored as a change in detector position for the dip in reflected intensity (the surface plasmon resonance phenomenon). Because the method strictly detects mass, there is no need to label the interacting components, thus eliminating possible changes of their molecular properties. We have utilized surface plasmon resonance to study the interaction of proteins of hair cells.