Use of respiratory quotient as a control parameter for optimum oxygen supply and scale‐up of 2,3‐butanediol production under microaerobic conditions
Use of respiratory quotient as a control parameter for optimum oxygen supply and scale‐up of 2,3‐butanediol production under microaerobic conditions
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DOI：
10.1002/bit.260440912
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发表时间：
1994-11
影响因子：
3.8
通讯作者：
A. Zeng;T. Byun;C. Posten;W. Deckwer
中科院分区：
文献类型：
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作者：
A. Zeng;T. Byun;C. Posten;W. Deckwer
呼吸商（RQ）被认为是微氧条件下生产2，3-丁二醇+乙偶姻的最佳供氧的合适控制参数。在实验室规模的连续培养中，在4.0至4.5之间的RQ值下获得2，3-丁二醇+乙偶姻的最佳生产。这与从所涉及的生物反应中化学计量得出的最佳RQ值（4.0）一致。在补料分批培养中，采用RQ供氧控制算法，可在32小时培养内实现高达102.9 g/L（96.0 g/L丁二醇+6.9 g/L乙偶姻）的产物浓度。在相似的条件下，在整个培养过程中控制恒定的供氧速率，仅获得85.7g/L产物（77.6g/L丁二醇+8.1g/L乙偶姻）。
The respiratory quotient (RQ) was found to be a suitable control parameter for optimum oxygen supply for the production of 2,3‐butanediol + acetoin under microaerobic conditions. In laboratory scale continuous cultures optimum production of 2,3‐butanediol + acetoin was obtained at an RQ value between 4.0 to 4.5. This agreed well with optimum RQ value (4.0) stoichiometrically derived from the bioreactions involved. In fed‐batch cultures product concentrations as high as 102.9 g/L (96.0 g/L butanediol + 6.9 g/L acetoin) can be achieved within 32 h cultivation with an RQ control algorithm for oxygen supply. Under similar conditions only 85.7 g/L product (77.6 g/L butanediol + 8.1 g/L acetoin) was obtained with control of constant oxygen supply rate throughout the cultivation.