Mechanism of expression of Thomsen-Friedenreich (T) antigen at the cell surface of a mammary adenocarcinoma.
Mechanism of expression of Thomsen-Friedenreich (T) antigen at the cell surface of a mammary adenocarcinoma.
复制标题
Thomsen-Friedenreich (T) 抗原在乳腺癌细胞表面的表达机制。
DOI：
10.1096/fasebj.2.8.3360239
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Carraway,KL
中科院分区：
文献类型：
--
作者：
Hull,SR;Carraway,KL
Thomsen-Friedenreich（T）抗原及其双糖成分Galβ1，3GalNAc（可被植物凝集素花生凝集素（PNA）识别）已被认为是有用的肿瘤标志物，因为它们在某些类型的癌症中明显特异性出现。我们使用脉冲追踪葡萄糖胺标记、蛋白水解和细胞表面唾液粘蛋白ASGP-1的PNA沉淀，研究了腹水13762大鼠乳腺癌细胞细胞表面出现二糖的机制。葡萄糖胺标记的二糖在不到10分钟的时间内出现在细胞表面。尽管出现的较大寡糖继续增加，但标记二糖的出现在一小时内趋于稳定。对细胞内与细胞表面标记的寡糖的分析表明，在到达细胞表面之前一个多小时合成的所有二糖都转化为更大的寡糖。因此，二糖在细胞表面的存在是由于其在唾液粘蛋白通过细胞表面的转运途径的后期合成。我们提出，癌细胞表面T抗原的存在是由于这些细胞中O-连接糖基化途径的异常，可能是由参与二糖合成的酶的不适当定位引起的。船体，S.的R.; Carraway，K. L. Mechanism of expression of Mesosen-Friedenreich（T）antigen at the cell surface of a mammary adenocarcinoma.FASEB J.2：2380 - 2384; 1988.
The Thomsen‐Friedenreich (T) antigen and its disaccharide component Galβ1,3GalNAc, which is recognized by the plant lectin peanut agglutinin (PNA), have been proposed as useful tumor markers because of their apparently specific occurrence in certain types of carcinomas. We have investigated the mechanism for the appearance of the disaccharide at the cell surface of ascites 13762 rat mammary adenocarcinoma cells using pulse‐chase glucosamine labeling, proteolysis, and PNA precipitation of the cell‐surface sialomucin ASGP‐1. Glucosamine‐labeled disaccharide appears at the cell surface in less than 10 min. Although the appearance of larger oligosaccharides continues to increase, the appearance of labeled disaccharide levels off within an hour. Analysis of intracellular vs. cell surface‐labeled oligosaccharides showed that all disaccharide synthesized more than an hour before reaching the cell surface is converted to larger oligosaccharides. Thus, the presence of the disaccharide at the cell surface results from its synthesis late in the transit pathway of the sialomucin tb the cell surface. We propose that the presence of T antigen at the surface of carcinoma cells results from an aberration of the pathway for O‐linked glycosyiation in these cells, probably caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.—Hull, S. R.; Carraway, K. L. Mechanism of expression of Thomsen‐Friedenreich (T) antigen at the cell surface of a mammary adenocarcinoma.FASEB J.2: 2380‐2384; 1988.