MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE STREPTAVIDIN GENE
MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE STREPTAVIDIN GENE
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DOI：
10.1093/nar/14.4.1871
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发表时间：
1986-02-25
影响因子：
14.9
通讯作者：
CANTOR, CR
中科院分区：
文献类型：
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作者：
ARGARANA, CE;KUNTZ, ID;CANTOR, CR
使用合成寡核苷酸作为探针，我们从阿维丁链霉菌基因组文库中克隆了链霉亲和素基因。核苷酸序列分析表明，2 Kb DNA 片段包含该基因的整个编码区、信号肽区以及 3' 和 5' 侧翼区。推导的氨基酸序列显示出与鸡蛋清亲和素的氨基酸序列有几个中断的同源性片段。对二级结构的分析表明，两种蛋白质中的β结构含量很高，并且它们之间的整体结构相当相似。
Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library ofStreptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3′ and 5′ flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.