NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
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NFIL3 作为核因子加速骨肉瘤进展
DOI：
10.1155/2019/4068521
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发表时间：
2019-11-30
影响因子：
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通讯作者：
Wang, Guangyou
中科院分区：
文献类型：
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作者：
Xu, Jiaxing;Xu, Gongping;Wang, Guangyou
目的骨肉瘤是最常见的原发恶性侵袭性骨肿瘤之一。然而，骨肉瘤增殖、迁移和侵袭的机制尚不清楚。为了探索骨肉瘤进展的可能机制，我们使用了一个公共数据库进行基因分析，以确定在骨肉瘤进展中重要的可能因素。核因子白介素3(NFIL3)在肉瘤组织中高表达。本研究旨在探讨NFIL3在骨肉瘤增殖、迁移和侵袭中的作用。方法采用逆转录聚合酶链式反应和免疫组织化学方法检测骨肉瘤组织中NFIL3的表达。为了阐明NFIL3在骨肉瘤中的作用，我们进行了细胞生长实验和克隆形成实验，以探讨NFIL3在骨肉瘤细胞增殖中的作用。此外，我们通过伤口愈合试验和跨孔迁移和侵袭试验分析了骨肉瘤细胞的迁移和侵袭。结果NFIL3在骨肉瘤组织中高表达，20例骨肉瘤组织中有15例高表达。体外实验证实，NFIL3能促进M6-63和SaOS2细胞的增殖(P<；0.01)。此外，NFIL3还促进了骨肉瘤细胞的迁移和侵袭(P<；0.05)。结论NFIL3在骨肉瘤组织中高表达，促进骨肉瘤细胞的增殖、迁移和侵袭。NFIL3有可能成为开发骨肉瘤新治疗策略的新靶点。
Purpose Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. Methods The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. Results NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). Conclusion NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma.