A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2
A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2
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DOI：
10.1073/pnas.96.8.4313
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发表时间：
1999-04-13
影响因子：
11.1
通讯作者：
Weissman, SM
中科院分区：
文献类型：
--
作者：
Liu, YC;Pan, J;Weissman, SM
最近在人类MHC I类区域发现了许多非I类基因。其中之一，FAT 10，编码由两个结构域组成的蛋白质，与泛素同源。FAT 10 mRNA在γ-干扰素诱导后在某些淋巴母细胞系和树突状细胞以及某些其他细胞中组成型表达。FAT 10蛋白表达在几个水平上受到控制，包括转录、翻译和蛋白稳定性。酵母双杂交筛选的人淋巴细胞库和免疫沉淀研究表明，FAT 10与MAD 2，一种蛋白质牵连在细胞周期检查点的纺锤体组装在后期非共价相关。因此，在B细胞或树突状细胞发育和活化期间，FAT 10可调节细胞生长。
Recently a number of nonclass I genes were discovered in the human MHC class I region. One of these, FAT10, encodes a protein consisting of two domains with homology to ubiquitin. FAT10 mRNA is expressed constitutively in some lymphoblastoid lines and dendritic cells and in certain other cells after gamma-interferon induction. FAT10 protein expression is controlled at several levels including transcription, translation, and protein stability. Yeast two-hybrid screening of a human lymphocyte library and immunoprecipitation studies revealed that FAT10 noncovalently associated with MAD2, a protein implicated in a cell-cycle checkpoint for spindle assembly during anaphase. Thus, FAT10 may modulate cell growth during B cell or dendritic cell development and activation.