Identification of sequences in the long terminal repeat of avian sarcoma virus required for efficient transcription.
Identification of sequences in the long terminal repeat of avian sarcoma virus required for efficient transcription.
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DOI：
10.1016/0042-6822(88)90415-1
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Siddarame Gowda;Arepalli S. Rao;Yong Woong Kim;R. Guntaka
中科院分区：
文献类型：
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作者：
Siddarame Gowda;Arepalli S. Rao;Yong Woong Kim;R. Guntaka
构建了两个不同的载体LTR-NEO-LTR和LTR-CAT-LTR，并在上游LTR的-299和-140处引入缺失。将这些缺失突变体在瞬时表达条件下引入QT 6细胞，并通过斑点杂交或CAT测定来监测转录水平。结果表明，RSV LTR的U3区中-208和-201之间以及-141和-119之间的核苷酸是有效转录所必需的。
Two different vectors, LTR-NEO-LTR and LTR-CAT-LTR, were constructed and deletions were introduced in the upstream LTR at −299 and at −140. These deletion mutants were introduced into QT6 cells under transient expression conditions, and the levels of transcription were monitored by dot blot hybridization or by CAT assays. The results indicate that the nucleotides between −208 and −201 and between −141 and −119 in the U3 region of RSV LTR are required for efficient transcription.