INVITRO SYNTHESIS OF APO-A-IV AND APO-C BY LIVER AND INTESTINAL MESSENGER-RNAS FROM LEAN AND OBESE ZUCKER RATS
INVITRO SYNTHESIS OF APO-A-IV AND APO-C BY LIVER AND INTESTINAL MESSENGER-RNAS FROM LEAN AND OBESE ZUCKER RATS
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DOI：
10.1016/0006-291x(87)90453-0
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发表时间：
1987-01-15
影响因子：
3.1
通讯作者：
INFANTE, R
中科院分区：
文献类型：
--
作者：
PESSAH, M;SALVAT, C;INFANTE, R
本文研究了肥胖高脂血症(FA/FA)和瘦(Fa/)Zucker大鼠肝脏和肠道细胞载脂蛋白A-IV和C基因的相对含量和表达。以35S蛋氨酸为标记物，在兔网织红细胞裂解系统中翻译RNA。用特异性抗体免疫沉淀载脂蛋白A-IV和载脂蛋白C，并用电泳法和放射自显影对其进行鉴定。肥胖大鼠肝脏中apo-A-IV和apo-C特异性基因的含量和表达水平明显高于瘦身大鼠。Fa/Fa和Fa/Fa大鼠肠细胞的apo-A-IV和apo-C合成能力相似，提示肥胖的Zucker大鼠的脂蛋白过量主要来源于肝脏。
The relative content and expression of mRNA coding for apolipoproteins A-IV and C in liver and intestinal cells have been studied in obese hyperlipemic (fa/fa) and lean (Fa/ ) Zucker rats. The RNA were translated in a rabbit reticulocyte lysate system using 35S methionine as label. Apo-A-IV and apo-C were immunoprecipitated with specific antibodies and characterized by electrophoresis and autoradiography. The content and expression of mRNA specific for apo-A-IV and apo-C was much higher and the liver of obese than of lean rats. The apo-A-IV and apo-C synthetic capacity of intestinal cells from fa/fa and Fa cells from fa/fa and Fa rats was similar suggesting that the lipoprotein overproduction, already described in obese Zucker rats, is mainly from hepatic origin.