CLONING OF A DELTA OPIOID RECEPTOR BY FUNCTIONAL EXPRESSION
CLONING OF A DELTA OPIOID RECEPTOR BY FUNCTIONAL EXPRESSION
复制标题
DOI：
10.1126/science.1335167
复制
发表时间：
1992-12-18
期刊：
影响因子：
56.9
通讯作者：
EDWARDS, RH
中科院分区：
文献类型：
--
作者：
EVANS, CJ;KEITH, DE;EDWARDS, RH
阿片类药物具有强效止痛和成瘾性。这些药物与受体相互作用，受体也介导对内源性阿片肽配体的反应。然而，阿片受体尚未得到明确的分子表征。通过在COS细胞中的瞬时表达和阿片肽脑啡肽的碘化类似物筛选，鉴定了一个编码功能性阿片受体的互补DNA克隆。该序列显示与G蛋白偶联受体同源，特别是生长抑素、血管紧张素和白细胞介素-8的受体。
Opiate drugs have potent analgesic and addictive properties. These drugs interact with receptors that also mediate the response to endogenous opioid peptide ligands. However, the receptors for opioids have eluded definitive molecular characterization. By transient expression in COS cells and screening with an iodinated analog of the opioid peptide enkephalin, a complementary DNA clone encoding a functional delta opioid receptor has been identified. The sequence shows homology to G protein-coupled receptors, in particular the receptors for somatostatin, angiotensin, and interleukin-8.