An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves
An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves
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DOI：
10.1038/s41467-019-13758-2
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发表时间：
2019-12-17
影响因子：
16.6
通讯作者：
Tanaka, Katsunori
中科院分区：
文献类型：
--
作者：
Vong, Kenward;Eda, Shohei;Tanaka, Katsunori
酶生物传感器是有用的工具，可以实时监测代谢物水平的快速变化。然而，目前的方法在很大程度上局限于有限的化学空间内的代谢物。随着人工金属酶（ArM）的不断发展，存在一个独特的机会，从根本上设计生物传感器的代谢物，是难以检测使用现有技术。在这里，我们提出了ArM乙烯探针（AEP）的设计和开发，其中白蛋白支架用于溶解和保护淬灭的钌催化剂。在植物激素乙烯的存在下，可以发生交叉复分解以产生荧光。该探针可用于检测果实和叶片中外源和内源诱导的乙烯生物合成变化。总的来说，这项工作代表了ArM生物传感器的一个例子，专门设计用于以前无法使用酶生物传感器的生物代谢物的空间和时间检测。
Enzyme biosensors are useful tools that can monitor rapid changes in metabolite levels in real-time. However, current approaches are largely constrained to metabolites within a limited chemical space. With the rising development of artificial metalloenzymes (ArM), a unique opportunity exists to design biosensors from the ground-up for metabolites that are difficult to detect using current technologies. Here we present the design and development of the ArM ethylene probe (AEP), where an albumin scaffold is used to solubilize and protect a quenched ruthenium catalyst. In the presence of the phytohormone ethylene, cross metathesis can occur to produce fluorescence. The probe can be used to detect both exo-genous- and endogenous-induced changes to ethylene biosynthesis in fruits and leaves. Overall, this work represents an example of an ArM biosensor, designed specifically for the spatial and temporal detection of a biological metabolite previously not accessible using enzyme biosensors.