Metabolomics of Mycobacterium tuberculosis.
Metabolomics of Mycobacterium tuberculosis.
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DOI：
10.1007/978-1-4939-2450-9_6
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Rhee, Kyu
中科院分区：
文献类型：
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作者：
Nandakumar, Madhumitha;Prosser, Gareth A;Rhee, Kyu
酶为所有细胞的生化活动提供燃料。因此，它们的底物和产物为了解细胞的生理状态提供了一个潜在的窗口。代谢组学侧重于对给定生物系统中小分子的全局或系统级研究，从而提供了在全球生化规模上研究细胞生理学的实验工具。虽然结核分枝杆菌的代谢组学研究仍处于起步阶段，但最近的研究已经开始对结核分枝杆菌生理网络的组成、组织、活性和调节提供独特的见解。在这里，我们概述了结核分枝杆菌代谢组样品培养、收集和分析的实用方法，强调最小的样品扰动、广泛和天然的代谢物回收以及灵敏的、生物学不可知的代谢物检测。
Enzymes fuel the biochemical activities of all cells. Their substrates and products thus offer a potential window into the physiologic state of a cell. Metabolomics focuses on the global, or systems-level, study of small molecules in a given biological system and thus provided an experimental tool with which to study cellular physiology on a global biochemical scale. While metabolomic studies of Mycobacterium tuberculosis are still in their infancy, recent studies have begun to deliver unique insights into the composition, organization, activity, and regulation of M. tuberculosis' physiologic network. Here, we outline practical methods for the culture, collection, and analysis of metabolomic samples from Mycobacterium tuberculosis that emphasize minimal sample perturbation, broad and native metabolite recovery, and sensitive, biologically agnostic metabolite detection.