Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements.
Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements.
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DOI：
10.1104/pp.88.1.92
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发表时间：
1988-09
期刊：
影响因子：
7.4
通讯作者：
Diane L. Church;A. Galston
中科院分区：
文献类型：
--
作者：
Diane L. Church;A. Galston
机械分离的百日草叶肉细胞在含有0.5微摩尔的α-萘乙酸和0.5微摩尔的苯基腺嘌呤的诱导培养基中培养时，可分化为导管分子。在生长素和/或细胞分裂素浓度已降至0.005微摩尔的培养液中，细胞不分化。细胞在培养72小时后，最初需要24小时暴露于诱导性细胞分裂素，56小时暴露于诱导性生长素才能分化。新分离的百日菊细胞在低浓度的生长素和细胞分裂素的培养液中只能维持1天，而在转移到诱导培养液中时不会明显丧失分化的潜力。在含有高生长素和低细胞分裂素或低生长素和高细胞分裂素的培养基中进行长达2天的初始培养，可以在转移到诱导培养基后的第三天充分分化，并促进某些细胞的早期分化。
Mechanically isolated mesophyll cells of Zinnia elegans L. cv Envy differentiate to tracheary elements when cultured in inductive medium containing 0.5 micromolar alpha-naphthaleneacetic acid and 0.5 micromolar benzyladenine. The cells do not differentiate when cultured in medium in which the concentration of auxin and/or cytokinin has been reduced to 0.005 micromolar. Cells require an initial 24-hour exposure to inductive cytokinin and 56-hour exposure to inductive auxin for differentiation at 72 hours of culture. Freshly isolated Zinnia cells can be maintained in medium having low concentrations of both auxin and cytokinin for only 1 day without significant loss of potential to differentiate upon transfer to inductive medium. Initial culture for up to 2 days in medium having high auxin and low cytokinin, or low auxin and high cytokinin, allows full differentiation on the third day after transfer to inductive medium and potentiates the early differentiation of some cells.