Traits of transgenic Atropa belladonna doubly transformed with different Agrobacterium rhizogenes strains
Traits of transgenic Atropa belladonna doubly transformed with different Agrobacterium rhizogenes strains
复制标题
不同发根农杆菌菌株双转化转基因颠茄的性状
DOI：
10.1007/bf00033885
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发表时间：
1994
影响因子：
3
通讯作者：
K. Shimomura
中科院分区：
文献类型：
--
作者：
M. Jaziri;K. Yoshimatsu;J. Homès;K. Shimomura
颠茄毛状根培养。分别用发根农杆菌atcc 15834或MAFF 03-01724进行培养，获得转基因植株。用MAFF 03-01724对转基因belladonnaplants （A. rhizogenes15834）叶片进行二次侵染，诱导双转化根。从双重转化的根中再生出芽和胎生叶。遗传转化是通过opine试验（agropine， mannopine和/或mikimopine）和聚合酶链反应来确定的。研究了毛状根（单转化和双转化）的生理变化和tropane生物碱的生物合成。与单转化根菌株相比，双转化根菌株的生物碱含量处于中等水平。另一方面，双转化根的内源IAA水平显著低于单转化根。
Hairy root cultures ofAtropa belladonnaL. were established by infection either withAgrobacterium rhizogenesATCC 15834 or MAFF 03-01724, and transgenic plants were obtained from both hairy root cultures. Doubly transformed roots were induced by re-infection of the leaf segments of transgenicAtropa belladonnaplants (A. rhizogenes15834) with MAFF 03-01724. Shoots and viviparous leaves were regenerated from the doubly transformed roots. The genetic transformation was determined by the opine assay (agropine, mannopine and/or mikimopine) and polymerase chain reaction. Physiological changes and tropane alkaloid biosynthesis in the hairy roots (singly and doubly transformed) were investigated. The alkaloid content in the doubly transformed root strain was intermediate as compared to the root strains which were singly transformed. On the other hand endogenous IAA levels in doubly transformed roots were significantly decreased compared to both singly transformed roots.