Analysis of chemotaxis in Dictyostelium.
Analysis of chemotaxis in Dictyostelium.
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DOI：
10.1007/978-1-61779-166-6_26
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Iijima, Miho
中科院分区：
文献类型：
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作者：
Cai, Huaqing;Huang, Chuan-Hsiang;Devreotes, Peter N;Iijima, Miho
盘形盘形骨是研究细胞定向迁移的良好模式生物，因为盘形盘形骨细胞对化学引诱剂cAMP表现出强烈的趋化反应。许多强大的实验工具是适用的，包括正向和反向遗传学，生物化学，显微镜，和蛋白质组学。最近的研究表明，许多参与趋化的成分在人类中性粒细胞和变形虫盘基骨柱之间功能上是保守的。在本节中，我们将描述如何定义盘基骨鞘在趋化过程中介导和调节细胞运动、细胞极性和方向传感的蛋白质的功能。
Dictyostelium discoideum is an excellent model organism for the study of directed cell migration since Dictyostelium cells show robust chemotactic responses to the chemoattractant cAMP. Many powerful experimental tools are applicable, including forward and reverse genetics, biochemistry, microscopy, and proteomics. Recent studies have demonstrated that many components involved in chemotaxis are functionally conserved between human neutrophils and Dictyostelium amoebae. In this section, we will describe how to define the functions of proteins that mediate and regulate cell motility, cell polarity, and directional sensing during chemotaxis in Dictyostelium.