Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.
Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.
复制标题
鉴定限制生殖细胞特异性基因转录的候选 c-mos 阻遏蛋白。
DOI：
10.1128/mcb.15.10.5369
复制
发表时间：
1995
影响因子：
5.3
通讯作者：
Cooper,GM
中科院分区：
文献类型：
--
作者：
Xu,W;Cooper,GM
c-mos原癌基因在雌性和雄性生殖细胞中特异性表达。以前的研究发现，c-mos启动子上游的负调控元件（NRE）抑制转染NIH 3 T3细胞中的c-mos转录。在这项研究中，我们使用凝胶迁移试验来检测NIH 3 T3细胞的核提取物中的蛋白质，这些蛋白质以序列特异性的方式与c-mosNRE结合。一种蛋白质被发现结合到NRE的一个区域，该区域通过定点诱变显示为抑制c-转录所需。该因子存在于几种体细胞系和组织的核提取物中，但不存在于c-mosis转录的雄性生殖细胞中，这表明它是c-mosis转录的体细胞阻遏物。c-mosNRE内候选阻遏物的结合位点由与在另外两个雄性生殖细胞特异性基因（编码鱼精蛋白2和磷酸甘油酸激酶2）中鉴定的推定NRE相关的序列组成。的c-mosrepressor绑定，并可以UV交联这些鱼精蛋白2和磷酸甘油酸激酶2基因序列作为一种蛋白质的表观分子量为;30 kDa。阻遏物结合位点在另外两个生殖细胞特异性基因（编码睾丸特异性细胞色素和热休克样蛋白70）中也是保守的，这表明c-mosrepressor可能通常参与抑制体细胞中生殖细胞特异性基因的转录。
The c-mosproto-oncogene is specifically expressed in female and male germ cells. Previous studies identified a negative regulatory element (NRE) upstream of the c-mospromoter that suppresses c-mostranscription in transfected NIH 3T3 cells. In this study, we used gel shift assays to detect proteins in nuclear extracts of NIH 3T3 cells that bind to the c-mosNRE in a sequence-specific manner. One protein was found to bind to a region of the NRE which was shown by site-directed mutagenesis to be required for suppression of c-mostranscription. This factor was present in nuclear extracts of several somatic cell lines and tissues but not in male germ cells in which c-mosis transcribed, suggesting that it is a somatic cell repressor of c-mostranscription. The binding site of the candidate repressor within the c-mosNRE consists of sequences related to putative NREs identified in two other male germ cell-specific genes (encoding protamine 2 and phosphoglycerate kinase 2). The c-mosrepressor bound and could be UV cross-linked to these protamine 2 and phosphoglycerate kinase 2 gene sequences as a protein with an apparent molecular mass of ;30 kDa. The repressor binding site is also conserved in two other germ cell-specific genes (encoding testis-specific cytochromecand heat shock-like protein 70), suggesting that the c-mosrepressor may be generally involved in suppressing transcription of germ cell-specific genes in somatic cells.