Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles
Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles
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DOI：
10.1016/j.jchromb.2004.01.050
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发表时间：
2004-07-25
影响因子：
3
通讯作者：
Mattiasson, B
中科院分区：
文献类型：
--
作者：
Plieva, FM;Savina, IN;Mattiasson, B
通过 AAm 与功能单体和交联剂 N,N'-亚甲基双丙烯酰胺 (MBAAm) 的自由基冷冻聚合（在中等冷冻体系中聚合）制备了基于超大孔整体丙烯酰胺 (AAm) 的冷冻凝胶。电子显微镜研究揭示了所开发的冷冻凝胶的超大孔结构，孔径为5-100μm。冷冻凝胶孔隙率取决于冷冻聚合条件。超过90%的整体床体积是充满水的互连的大孔，不到10%的整体体积是孔壁。总蛋白结合能力（固定金属亲和层析 (IMAC) 柱中的溶菌酶和阴离子交换 (AE) 柱中的牛血清白蛋白 (BSA)）在 600 cm/h 之前与流速无关。当细胞悬浮液应用于离子交换冷冻凝胶柱时，大肠杆菌细胞的色谱行为取决于功能配体的密度和冷冻凝胶的孔隙率。 (C) 2004 Elsevier B.V. 保留所有权利。
Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (C) 2004 Elsevier B.V. All rights reserved.