High-performance liquid chromatography with ultraviolet detection for real-time therapeutic drug monitoring of meropenem in plasma
High-performance liquid chromatography with ultraviolet detection for real-time therapeutic drug monitoring of meropenem in plasma
复制标题
DOI：
10.1016/j.jchromb.2007.05.043
复制
发表时间：
2007-09-01
影响因子：
3
通讯作者：
Kobayashi, Masao
中科院分区：
文献类型：
--
作者：
Ikeda, Kayo;Ikawa, Kazuro;Kobayashi, Masao
开发了一种简单、快速、精确的高效液相色谱 (HPLC) 方法，使用超滤去除血浆蛋白，用于在临床环境中测定人血浆中美罗培南的浓度。通过在肝素化真空管中收集的血液在4℃下离心分离血浆，并通过立即将血浆与1M 3-吗啉代丙磺酸缓冲液(pH 7.0)(1:1)混合来稳定美罗培南。超滤用于血浆脱蛋白。通过 300 nm 处的紫外吸光度检测美罗培南，无干扰血浆峰。人血浆中美罗培南的校准曲线在0.05至100μg/mL之间呈线性。日内、日间精密度均小于7.17%(CV)，0.05μg/mL准确度在97.7%～106.3%之间。检测限为0.01μg/mL。该测定已在临床上应用于儿科患者的实时治疗药物监测和药代动力学研究。 (C) 2007 Elsevier B.V. 保留所有权利。
A simple, rapid, and precise high-performance liquid chromatography (HPLC) method using ultrafiltration to remove plasma protein was developed to determine meropenem concentrations in human plasma in a clinical setting. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and meropenem was stabilized by immediately mixing the plasma with I M 3-morpholinopropanesulfonic acid buffer (pH 7.0) (1:1). Ultrafiltration was used for plasma deproteinization. Meropenem was detected by ultraviolet absorbance at 300 nm with no interfering plasma peak. The calibration curve of meropenem in human plasma was linear from 0.05 to 100 mu g/mL. Intraday and interday precision was less than 7.17% (CV), and accuracy was between 97.7% and 106.3% over 0.05 mu g/mL. The limit of detection was 0.01 mu g/mL. The assay has been clinically applied to a real-time therapeutic drug monitoring in pediatric patients and pharmacokinetic studies. (C) 2007 Elsevier B.V. All rights reserved.