FORMATION AND GROWTH OF MULTICELLULAR SPHEROIDS OF HUMAN-ORIGIN
FORMATION AND GROWTH OF MULTICELLULAR SPHEROIDS OF HUMAN-ORIGIN
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DOI：
10.1002/ijc.2910310502
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
COLLINS, VP
中科院分区：
文献类型：
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作者：
CARLSSON, J;NILSSON, K;COLLINS, VP
测试了通常以单层或悬浮培养物形式生长的不同类型的人类细胞形成和生长为球体的能力。 27 个测试的肿瘤细胞系中有 16 个形成球状体。几乎所有这些球体也都长大了。除 2 个例外外，肿瘤细胞以球体形式生长时的倍增时间比在传统大规模培养中生长时的倍增时间更长。 13 个测试的人类非肿瘤细胞中有 11 个形成了小球体，但其中只有淋巴来源的球体可以生长。这些淋巴细胞聚集成球体时比单细胞悬浮培养时生长得更快。通常以单层形式生长的其他非肿瘤细胞都不能以球体形式生长。正常单层培养的肿瘤细胞形成具有光滑表面的对称球体，而正常悬浮培养的细胞形成具有粗糙表面的不规则球体。所有大球体都有一个坏死中心，周围环绕着一层活细胞。活细胞层的厚度根据细胞类型而变化。球体内细胞的形状和组织也有很大差异。许多类型的人类细胞可以培养为球体，并且可以获得广泛的形态外观和生长速率。
Different types of human cells which normally grow as monolayers or suspension cultures were tested for their capacity to form and grow as spheroids. Sixteen out of the 27 tested tumor cell lines formed spheroids. Nearly all of these spheroids also grew. With only 2 exceptions the doubling times were longer when the tumor cells grew as spheroids than when they grew in conventional mass culture. Eleven out of 13 tested human non-tumor cells formed small spheroids but of these only the spheroids of lymphoid origin could grow. These lymphoid cells grew faster when aggregated to spheroids than when in single-cell suspension culture. None of the other non-tumor cells, which normally grew as monolayers, could grow as spheroids. The normally monolayer-cultured tumor cells formed symmetrical spheroids with smooth surfaces while the normally suspension-cultured cells formed irregular spheroids with rough surfaces. All large spheroids had a necrotic center surrounded by a shell of viable cells. The thickness of the viable cell layer varied depending on cell type. The shape and organization of cells within the spheroids also varied largely. Many types of human cells can be cultured as spheroids and a wide spectrum of morphological appearances and growth rates can be obtained.