A FLOW SYSTEM FOR THE STUDY OF SHEAR FORCES UPON CULTURED ENDOTHELIAL-CELLS
A FLOW SYSTEM FOR THE STUDY OF SHEAR FORCES UPON CULTURED ENDOTHELIAL-CELLS
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DOI：
10.1115/1.3138625
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发表时间：
1986-11-01
影响因子：
1.7
通讯作者：
SCHUSTER, P
中科院分区：
文献类型：
--
作者：
KOSLOW, AR;STROMBERG, RR;SCHUSTER, P
设计了一种流动系统中的平行板腔室，用于研究流体剪切应力对细胞的影响。该系统被应用于对培养在盖玻片上的内皮细胞的研究，盖玻片放置在底板的凹槽内。腔室内的染料注入研究表明细胞上方为层流。通过电化学技术测量盖玻片上方的剪切速率，发现其与流速呈线性关系。激光多普勒测速仪显示板间为抛物线型剖面。受到流动作用的内皮细胞在取向所需时间和剪切应力大小之间呈现出相关性。
A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber indicated laminar flow over the cells. Shear rates measured over the cover slips by an electrochemical technique were found to be linear with flow rate. Laser doppler anemometry showed parabolic profiles between the plates. Endothelial cells subjected to flow showed a correlation between the time required for orientation and the magnitude of the shear stress.