MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line.
MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line.
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Zbtb1 基因敲除单克隆 EL4 细胞系中的 MicroRNA 和 circRNA 表达分析
DOI：
10.3389/fcimb.2021.706919
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发表时间：
2021
影响因子：
5.7
通讯作者：
Wang CF
中科院分区：
文献类型：
--
作者：
Wang JH;Shi CW;Lu YY;Zeng Y;Cheng MY;Wang RY;Sun Y;Jiang YL;Yang WT;Zhao DD;Huang HB;Ye LP;Cao X;Yang GL;Wang CF
Zbtb 1（Zinc finger and BTB domain containing 1）是一种转录抑制蛋白，属于哺乳动物Zbtb基因家族。先前的研究表明，Zbtb 1对T细胞发育至关重要。然而，Zbtb 1在T细胞淋巴瘤中的作用尚未确定。在这项研究中，使用CRISPR-Cas9技术构建了Zbtb 1缺失的EL 4细胞系。通过RNA-seq确定对照组和基因缺失组产生的microRNA和circRNA的表达谱。总的来说，在正常组和基因缺失组中，发现24个microRNA和16个circRNA的差异表达。通过进一步分析差异表达基因，绘制GO term直方图和KEGG散点图，发现3对miRNA和circRNA调控关系，本研究描述了正常和Zbtb 1缺陷EL 4细胞系中差异表达的microRNA和circRNA，从而为T细胞淋巴瘤的药物开发和临床治疗提供潜在靶点。
Zinc finger and BTB domain containing 1(Zbtb1) is a transcriptional suppressor protein, and a member of the mammalian Zbtb gene family. Previous studies have shown that Zbtb1 is essential for T-cell development. However, the role of Zbtb1 in T-cell lymphoma is undetermined. In this study, an EL4 cell line with Zbtb1 deletion was constructed using the CRISPR-Cas9 technique. The expression profiles of microRNA and circRNA produced by the control and gene deletion groups were determined by RNA-seq. In general, 24 differentially expressed microRNA and 16 differentially expressed circRNA were found between normal group and gene deletion group. Through further analysis of differentially expressed genes, GO term histogram and KEGG scatter plot were drawn, and three pairs of miRNA and circRNA regulatory relationships were found. This study describes the differentially expressed microRNA and circRNA in normal and Zbtb1-deficient EL4 cell lines, thus providing potential targets for drug development and clinical treatment of T-cell lymphoma.