Determination of the infectious dose of Helicobacter pylori during primary and secondary infection in rhesus monkeys (Macaca mulatta)
Determination of the infectious dose of Helicobacter pylori during primary and secondary infection in rhesus monkeys (Macaca mulatta)
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DOI：
10.1128/iai.69.11.6887-6892.2001
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发表时间：
2001-11-01
影响因子：
3.1
通讯作者：
Parsonnet, J
中科院分区：
文献类型：
--
作者：
Solnick, JV;Hansen, LM;Parsonnet, J
我们试图确定恒河猴原发和继发感染幽门螺杆菌的感染剂量，并确定接种前抑酸是否是产生定植所必需的。将三株人源菌株混合接种，结果表明，H。pylori J166特别适合于恒河猴的定殖，因为它胜过了两种其它菌株。H. pylori J166为10（4）株特异性致病菌（H. pylori）-free猴。抗生素治疗后这些猴子的再激发的特征是与原发感染相比细菌负荷减少10至100倍，但感染剂量几乎没有变化。接种前的抑酸不是定植发生所必需的。这些结果为未来使用更生态相关的接种条件进行动物实验提供了基础，并表明减少细菌负荷而不是完全保护可能是H.幽门疫苗。
We sought to determine the infectious dose of Helicobacter pylori during primary and secondary infection in the rhesus monkey and to determine whether preinoculation acid suppression is necessary to produce colonization. Mixed inoculation with three human-derived strains showed that H. pylori J166 is particularly adapted to colonization of rhesus monkeys, since it outcompeted two other strains. The minimum infectious dose of H. pylori J166 was 10(4) bacteria in specific-pathogen (H. pylori)-free monkeys. Rechallenge of these monkeys after antibiotic therapy was characterized by a 10- to 100-fold decrease in bacterial load compared to primary infection, but with little change in the infectious dose. Acid suppression prior to inoculation was not necessary for colonization to occur. These results provide a basis for future animal experiments using more ecologically relevant conditions of inoculation and suggest that reduction in bacterial load rather than complete protection may be a more realistic goal for H. pylori vaccination.