Purification of Substances from Achyrocline satureioides with Inhibitory Activity Against Paenibacillus larvae, the Causal Agent of American Foulbrood in Honeybees' Larvae
Purification of Substances from Achyrocline satureioides with Inhibitory Activity Against Paenibacillus larvae, the Causal Agent of American Foulbrood in Honeybees' Larvae
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DOI：
10.1007/s12010-015-1506-5
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发表时间：
2015-04-01
影响因子：
3
通讯作者：
Marioli, Juan M.
中科院分区：
文献类型：
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作者：
Gonzalez, Maria J.;Beoletto, Viviana G.;Marioli, Juan M.
采用体外抗芽孢杆菌生长的实验方法，研究了水蛭素提取物对芽孢杆菌幼虫生长的抑制作用。四种不同的提取物通过液-液萃取从水-乙醇浸渍植物的地上部分。采用微量肉汤稀释法测定其生物活性。正己烷提取物的抑菌活性最高，最低抑菌浓度为0.060 +/- 0.037 mg/mL。透射电镜实验表明，己烷提取物对细胞的作用主要在细胞膜水平。采用薄层色谱法对正己烷提取物进行分析，采用生物自显影法检测其活性。生物自显影实验（以正己烷-丙酮（7:3）为流动相）观察到四个生长抑制区，其中Rf = 0.57时抑制区最大。高效液相色谱实验，采用紫外和电喷雾电离耦合串联质谱检测，发现了一个m/z比为442的化合物，该化合物可能与最近发现的间苯三酚-吡咯酮化合物有关。己烷萃取物和分离物具有较高的抑菌活性，为今后开发处理美国恶臭菌的新方法提供了参考。
Achyrocline satureioides extracts were tested in vitro against the growth of Paenibacillus larvae. Four different extracts were obtained by liquid-liquid extraction from an aqueous-ethyl alcohol macerate of the aerial parts of the plant. The biological activity was tested by the broth microdilution technique. Hexane extract showed the highest activity (minimum inhibitory concentration = 0.060 +/- 0.037 mg/mL). Transmission electron microscopy experiments showed that the main effect exerted by the hexane extract on the cell was at the cellular membrane level. The hexane extract was analyzed by thin-layer chromatography, and the activity of its components was tested by bioautography. Four growth inhibition zones were observed in the bioautographic experiments (using hexane-acetone (7:3) as mobile phase), with that at Rf = 0.57 showing the largest zone of inhibition. High-performance liquid chromatographic experiments, using ultraviolet and electrospray ionization coupled to tandem mass spectrometric detection, showed the presence of one compound with a m/z ratio of 442, which may be related to phloroglucinols alpha-pyrone compounds recently discovered. The high antibacterial activity of the hexane extract and of the isolated compound determined in this work may be useful for the development of future new alternatives for the treatment of American foulbrood.