A computer system for combined neuronal mapping and morphometry.
A computer system for combined neuronal mapping and morphometry.
复制标题
用于组合神经元映射和形态测量的计算机系统。
DOI：
10.1016/0165-0270(81)90038-8
复制
发表时间：
1981
影响因子：
3
通讯作者：
SloanJr,KR
中科院分区：
文献类型：
--
作者：
Curcio,CA;SloanJr,KR
已经开发了一种计算机系统，可以对组织学准备进行制图和平面测量。数据包括在厚切片的低倍显微镜下定义的细胞结构边界，以及在原始厚组织的半薄切片的高倍显微镜下定义的核和血管轮廓，以及两者共同的标志。从这些不同的准备和放大的数据被输入到数字化平板电脑上的计算机中，并在现实世界的坐标系统中作为点存储，同时存储的还有剖面标签、用于校准的地标和剖面深度。随后的程序确定了每个剖面的面积和周长等参数，以及其几何中心和与细胞结构边界的关系。该映射参数允许将剖面作为其位置的函数进行形态计量分析。其他程序允许显示和操作三维数据、细胞计数和立体学。数据采集是在实验室中使用微型计算机完成的；分析和显示是在一台大型分时计算机上完成的，该计算机通过电话接口与小型机器相连。
A computer system has been developed which allows both mapping and planimetry of histological preparations. Data consist of cytoarchitectonic boundaries defined in low-power micrographs of thick sections, and nuclear and vascular profiles defined in high-power micrographs of semi-thin sections taken from the original thick tissue, and landmarks common to both. Data from these different preparations and magnifications are entered into the computer on a digitizing tablet and are stored as points in a real-world coordinate system, along with profile labels, landmarks for alignment purposes, and section depth. Subsequent programs determine parameters such as area and perimeter for each profile, as well as its geometric center and relationship to the cytoarchitectonic boundary. This mapping parameter allows morphometric analysis of profiles as a function of their position. Other programs allow display and manipulation of data in 3 dimensions, cell counts, and stereology. Data acquisition is done in the laboratory, using a micro-computer; analysis and display are done on a large time-sharing computer connected to the small machine by a telephone interface.