CELL-CELL INTERACTIONS IN CONJUGATING ESCHERICHIA-COLI - ROLE OF TRAT PROTEIN IN SURFACE EXCLUSION
CELL-CELL INTERACTIONS IN CONJUGATING ESCHERICHIA-COLI - ROLE OF TRAT PROTEIN IN SURFACE EXCLUSION
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DOI：
10.1073/pnas.74.11.5104
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
SKURRAY, R
中科院分区：
文献类型：
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作者：
ACHTMAN, M;KENNEDY, N;SKURRAY, R
E.携带F性因子的大肠杆菌细胞是接合不良的受体。这种现象称为表面排斥。两个F顺反子，traS和traT，独立地负责整个机制的一部分。traS基因产物减少稳定交配聚集体内的DNA转移。traT基因产物pTraT导致形成稳定交配聚集体的能力大大降低，因此也导致细胞群内DNA转移减少。pTraT是掺入细胞包膜外膜的25，000道尔顿蛋白。根据表达它的质粒不同，它的拷贝数为29，000 - 84，000/细胞。表面排斥模型进行了讨论。
E. coli cells carrying the F sex factor are poor recipients in conjugation. This phenomenon is called surface exclusion. Two F cistrons, traS and traT, are independently responsible for part of the whole mechanism. The traS gene product reduces DNA transfer within stable mating aggregates. The traT gene product, pTraT, results in a greatly reduced ability to form stable mating aggregates, and thus also leads to reduced DNA transfer within the cell population. pTraT is a 25,000 dalton protein incorporated into the cell envelope outer membrane. It is found in 29,000-84,000 copies/cell, depending on the plasmid expressing it. There is a parallel variation in recipient ability. Models for surface exclusion are discussed.