Mucosal immunization with polymeric antigen BLSOmp31 using alternative delivery systems against Brucella ovis in rams
Mucosal immunization with polymeric antigen BLSOmp31 using alternative delivery systems against Brucella ovis in rams
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DOI：
10.1016/j.vetimm.2019.02.005
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发表时间：
2019-03-01
影响因子：
1.8
通讯作者：
Marcela Estein, Silvia
中科院分区：
文献类型：
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作者：
Graciela Diaz, Alejandra;Alejandra Quinteros, Daniela;Marcela Estein, Silvia
亚细胞疫苗可以解决羊种布氏杆菌Rev 1的一些缺点。我们已经证明，用乳化在油佐剂中的多聚抗原BLSOmp 31的肠胃外免疫赋予针对B的显著保护。羊在公羊。在我们以前的研究中，我们已经表征了壳聚糖微球（ChMs）和温敏性和粘膜粘附性原位凝胶（泊洛沙姆407-Ch）作为用于在鼻和结膜粘膜中递送BLSOmp 31的两种新的制剂策略。在目前的工作中，我们评估了免疫原性和保护所赋予的鼻内和结膜免疫与这两个粘膜传递系统对B。羊在公羊。通过鼻内途径施用的BLSOmp 31-ChM和通过鼻内或结膜途径施用的BLSOmp 31-P407-Ch诱导全身、局部和包皮IgG和伊加抗体应答。两种制剂均未显示对血清学诊断的干扰。因此，使用任一制剂的粘膜免疫诱导显著的特异性细胞免疫应答（体外和体内），并且其防止B的排泄。精液中的绵羊虽然这些疫苗不能预防免疫公羊的感染，但接种疫苗和未接种疫苗公羊之间感染器官的定殖减少和细菌分布存在显著差异。
Subcellular vaccines against ovine contagious epididymitis due Brucella ovis can solve some shortcomings associated with the use of Brucella melitensis Rev 1. We have demonstrated that the parenteral immunization with polymeric antigen BLSOmp31 emulsified in oil adjuvant conferred significant protection against B. ovis in rams. In our previous studies, we have characterized chitosan microspheres (ChMs) and a thermoresponsive and mucoadhesive in situ gel (Poloxamer 407-Ch) as two novel formulation strategies for the delivery of BLSOmp31 in nasal as well as conjunctival mucosa. In the present work, we evaluated the immunogenicity and protection conferred by the intranasal and conjunctival immunization with these two mucosal delivery systems against B. ovis in rams. BLSOmp31-ChM administered by intranasal route and BLSOmp31-P407-Ch applied by intranasal or conjunctival routes induced systemic, local and preputial IgG and IgA antibody response. Neither formulation showed interference in the serological diagnosis. Thus, mucosal immunization using either formulation induced significant specific cellular immune responses (in vitro and in vivo) and it prevented the excretion of B. ovis in semen. Although these vaccines did not prevent infection in immunized rams, colonization reduction of infected organs and bacterial distribution differed significantly between vaccinated and unvaccinated rams.