Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.
Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.
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Rolosense：使用基于 DNA 的电机对 SARS-CoV-2 进行机械检测。
DOI：
10.1101/2023.02.27.530294
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Salaita,Khalid
中科院分区：
文献类型：
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作者：
Piranej,Selma;Zhang,Luona;Bazrafshan,Alisina;Marin,Mariana;Melikyan,GregoryB;Salaita,Khalid
检测病毒感染的检测方法在限制SARS-CoV-2等疾病的传播方面发挥着重要作用。在这里，我们提出了一种病毒传感平台Rolosense，它利用RNA燃料芯片上5 μm dna马达的运动来转导病毒的存在。马达和芯片用适体修饰，适体设计用于与病毒靶标多价结合并导致运动停滞。因此，马达对病毒靶标进行“机械测试”，并在整个病毒粒子存在的情况下停止，这代表了与传统检测不同的独特转导机制。Rolosense可以检测人工唾液和呼出冷凝水中添加的SARS-CoV-2，灵敏度为103拷贝/mL，并与其他呼吸道病毒区分。正如我们对甲型流感和SARS-CoV-2的一锅检测所证明的那样，该检测方法是模块化的，适合多路复用。作为概念验证，我们表明可以使用带有显微镜附件的智能手机相机在短短15分钟内实现读数，而无需放大反应。综上所述，这些结果表明，使用Rolosense的机械检测可以广泛应用于任何病毒靶点，并有可能实现快速、低成本的循环病毒即时筛查。
Assays that detect viral infections play a significant role in limiting the spread of diseases such as SARS-CoV-2. Here, we present Rolosense, a virus sensing platform that leverages the motion of 5 μm DNA-based motors on RNA fuel chips to transduce the presence of viruses. Motors and chips are modified with aptamers, which are designed for multivalent binding to viral targets and lead to stalling of motion. Therefore, the motors perform a “mechanical test” of the viral target and stall in the presence of whole virions, which represents a unique mechanism of transduction distinct from conventional assays. Rolosense can detect SARS-CoV-2 spiked in artificial saliva and exhaled breath condensate with a sensitivity of 103copies/mL and discriminates among other respiratory viruses. The assay is modular and amenable to multiplexing, as demonstrated by our one-pot detection of influenza A and SARS-CoV-2. As a proof of concept, we show that readout can be achieved using a smartphone camera with a microscopic attachment in as little as 15 min without amplification reactions. Taken together, these results show that mechanical detection using Rolosense can be broadly applied to any viral target and has the potential to enable rapid, low-cost point-of-care screening of circulating viruses.