DNA sequences that mediate attenuation of transcription from the mouse protooncogene myc.
DNA sequences that mediate attenuation of transcription from the mouse protooncogene myc.
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介导小鼠原癌基因 myc 转录减弱的 DNA 序列。
DOI：
10.1073/pnas.86.2.505
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发表时间：
1989
影响因子：
11.1
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Wright,S;Bishop,JM
原癌基因myc的表达受多种机制的调控，可能参与细胞增殖的调控。在正常细胞和肿瘤细胞中，通过myc基因外显子1的衰减来调节转录延长被认为是决定myc RNA水平的重要因素。我们发现，小鼠myc的第一个外显子包含可能介导转录衰减的特定DNA序列。来自外显子1 3‘端的180个核苷酸的DNA片段被放置在人α1-珠蛋白基因的内含子内时，减少了聚合酶II的转录延伸，并通过转染HeLa细胞进行了检测。一个36个核苷酸的序列，类似于各种转录终止信号，位于该myc片段中，但其本身不足以在置于α-珠蛋白基因内时引起衰减。因此，通过基因中特定的DNA序列来调节转录延长可以提供一种调节其表达的机制。
Expression of the protooncogene myc is regulated by multiple mechanisms and is probably involved in the control of cellular proliferation. Modulation of transcriptional elongation by attenuation within exon 1 of the myc gene is thought to play an important role in determining levels of myc RNA in both normal and tumor cells. We show that the first exon of mouse myc contains specific DNA sequences that may mediate transcriptional attenuation. A 180-nucleotide DNA fragment derived from the 3' end of exon 1 reduced transcriptional elongation by polymerase II when placed within an intron of the human alpha 1-globin gene and assayed by transfection into HeLa cells. A 36-nucleotide sequence that resembles a variety of transcriptional termination signals was located within this myc fragment but was by itself insufficient to cause attenuation when placed within the alpha-globin gene. Modulation of transcriptional elongation through specific DNA sequences within a gene may thus provide a mechanism for regulating its expression.