Effect of mupirocin on the growth and lifespan of human fibroblasts.
Effect of mupirocin on the growth and lifespan of human fibroblasts.
复制标题
莫匹罗星对人成纤维细胞生长和寿命的影响。
DOI：
10.1111/1523-1747.ep12470407
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Carter,DM
中科院分区：
文献类型：
--
作者：
Balin,AK;Leong,I;Carter,DM
我们研究了抗生素莫匹罗星对人成纤维细胞生长和增殖寿命的影响。人胚肺成纤维细胞在含有 10% 胎牛血清和 0.5-756 μg/ml 不同浓度莫匹罗星的 Dulbecco 基本培养基中生长。对照培养物与在 0.5 μg、72 μg 或 76 μg/ml 莫匹罗星中生长的培养物之间的指数生长速率和最终达到的饱和密度几乎没有差异。在含有 650-750 μg/ml 莫匹罗星的培养物中，生长被延缓或抑制。在含有 7 μg/ml 或 100 μg/mi 莫匹罗星的培养基中连续传代培养的培养物中检查了成体皮肤成纤维细胞的增殖寿命。在这些莫匹罗星浓度下培养的培养物能够充分发挥其增殖潜力。这项研究表明，尽管 700 μg/ml 莫匹罗星会抑制人成纤维细胞的生长，但在至少 100 μg/ml 莫匹罗星存在的情况下，人成纤维细胞的生长和增殖寿命不会改变。这些观察结果表明，长期使用低浓度但具有杀菌作用的莫匹罗星不会损害愈合伤口中成纤维细胞的生长。
We have examined the effect of the antibiotic mupirocin on the growth and proliferative lifespan of human fibroblasts. Human embryonic lung fibroblasts were grown in Dulbecco's minimal essential medium, containing 10% fetal bovine serum and various concentrations of mupirocin ranging from 0.5–756 μg/ml. There is little difference in the rate of exponential growth and the final saturation density reached between control cultures and those grown in 0.5 μg, 72 μg, or 76 μg/ml mupirocin. Growth was retarded or inhibited in cultures containing 650–750 μg/ml mupirocin. The proliferative lifespan of adult skin fibroblasts was examined in cultures serially subcultivated in medium containing either 7 μg/ml or 100 μg/mi mupirocin. Cultures incubated in these concentrations of mupirocin were capable of reaching their full proliferative potential. This study shows that the growth and proliferative lifespan of human fibroblasts is unaltered in the presence of at least 100 μg/ml mupirocin although growth of human fibroblasts is inhibited by 700 μg/ml mupirocin. These observations suggest that long-term use of low but bactericidal concentrations of mupirocin will not impair growth of fibroblasts in healing wounds.