Cathelicidin-NV from Nanorana ventripunctata effectively protects HaCaT cells, ameliorating ultraviolet B-induced skin photoaging
Cathelicidin-NV from Nanorana ventripunctata effectively protects HaCaT cells, ameliorating ultraviolet B-induced skin photoaging
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Nanorana ventripunctata 中的 Cathelicidin-NV 有效保护 HaCaT 细胞，改善紫外线 B 引起的皮肤光老化
DOI：
10.1016/j.peptides.2021.170712
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发表时间：
2021-12-29
期刊：
影响因子：
3
通讯作者：
Mu, Lixian
中科院分区：
文献类型：
--
作者：
Feng, Guizhu;Wei, Lin;Mu, Lixian
放线菌素是脊椎动物免疫系统中的多种效应分子，与免疫调节、炎症反应、伤口愈合和血管形成有关。然而，人们对它们清除自由基的能力知之甚少，特别是在体内。在这项研究中，我们从斑腹高原蛙(无尾无尾轮虫，双舌蛙科，双舌蛙亚科)中鉴定出一种新的长春新素分子，ARGKECKDDRCRLMKRGSFSYV，它可以缓解中波紫外线(UVB)诱导的小鼠皮肤光老化。长春花碱能有效抑制UVB辐射诱导的HaCaT细胞的细胞毒作用、DNA断裂和细胞凋亡，并降低参与调节胶原降解的JNK、c-Jun和MMP1的蛋白表达水平。此外，中草药还可清除UVB诱导的细胞内活性氧(ROS)。综上所述，放线菌素-NV直接清除细胞内过量的ROS以保护HaCaT细胞，从而缓解UVB诱导的皮肤光老化。这项研究扩大了有关长春花素家族抗氧化功能的报道。此外，长春花素-NV的特性使其成为预防和治疗紫外线诱导的皮肤光老化的极佳候选药物。
Cathelicidins are diverse effector molecules in the vertebrate immune system and are related to immune regulation, inflammatory response, wound healing, and blood vessel formation. However, little is known about their free radical scavenging ability, especially in vivo. In this study, a cathelicidin molecule (cathelicidin-NV, ARGKKECKDDRCRLLMKRGSFSYV) previously identified from the spot-bellied plateau frog (Nanorana ventripunctata) (Anura, Dicroglossidae, Dicroglossinae) by us was shown to alleviate ultraviolet B (UVB)-induced skin photoaging in mice. Cathelicidin-NV effectively suppressed cytotoxicity, DNA fragmentation, apoptosis and reduced the protein expression levels of JNK, c-Jun, and MMP-1, which are involved in the regulation of collagen degradation in HaCaT cells induced by UVB irradiation. Furthermore, cathelicidin-NV also scavenged UVBinduced intracellular reactive oxygen species (ROS). Taken together, cathelicidin-NV directly scavenged excessive intracellular ROS to protect HaCaT cells, and subsequently alleviated UVB-induced skin photoaging. This study extends reports on the antioxidant function of the cathelicidin family. In addition, the properties of cathelicidin-NV make it an excellent candidate for the prevention and treatment of UV-induced skin photoaging.