PYRAMIDAL NEURONS OF THE RAT CEREBRAL-CORTEX, IMMUNOREACTIVE TO NICOTINIC ACETYLCHOLINE-RECEPTORS, PROJECT MAINLY TO SUBCORTICAL TARGETS
PYRAMIDAL NEURONS OF THE RAT CEREBRAL-CORTEX, IMMUNOREACTIVE TO NICOTINIC ACETYLCHOLINE-RECEPTORS, PROJECT MAINLY TO SUBCORTICAL TARGETS
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DOI：
10.1002/cne.903200104
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发表时间：
1992-06-01
影响因子：
2.5
通讯作者：
KARTEN, HJ
中科院分区：
文献类型：
--
作者：
BRAVO, H;KARTEN, HJ
用针对乙酰胆碱结合亚基（α4）或乙酰胆碱结构亚基（β2）的单克隆抗体对大鼠大脑中对烟碱型乙酰胆碱受体（nAChR）有免疫反应的皮质神经元进行了鉴定。在所有皮质区域都发现了异质性的nAChR - LI神经元群体。最显著的免疫反应神经元是V层和II - III层的锥体神经元。非锥体阳性神经元是VIb层的水平梭形神经元、I层的小细胞以及II - V层的圆形或卵圆形神经元。双标记实验（免疫组织化学和荧光逆行示踪剂）表明，V层的胆碱能感受性锥体神经元主要投射到皮质下靶点，如尾状核 - 壳核、上丘和脑桥核，而极少数nAChR阳性神经元连接到其他皮质区域。这些发现表明，通常认为的胆碱能神经支配对皮质神经元的兴奋性作用可能对皮质 - 皮质下输出的影响比对皮质 - 皮质间的影响更大。
Cortical neurons immunoreactive to nicotinic acetylcholine receptors (nAChR) of the rat brain were characterized with monoclonal antibodies directed to ACh-binding subunits (alpha 4) or to ACh-structural subunits (beta2). A heterogeneous population of nAChR-LI neurons was found in all cortical regions. The most prominent immunoreactive neurons were pyramids of layers V and II-III. The nonpyramidal positive neurons were fusiform horizontally oriented neurons of layer VIb, small cells of layer I and round or ovoid neurons of layers II-V.Double labeled experiments (immunohistochemistry and fluorescent retrograde tracers) showed that cholinoceptive pyramidal neurons of layer V project mainly to subcortical targets such as caudate-putamen, superior colliculus, and pontine nuclei, while very few nAChR positive neurons connect to other cortical areas. These findings suggest that the mainly excitatory effect that has been attributed to the cholinergic innervation upon the cortical neurons may have a greater influence upon the cortico-subcortical output than the cortico-cortical one.