AM-3K, an anti-macrophage antibody, recognizes CD163, a molecule associated with an anti-inflammatory macrophage phenotype
AM-3K, an anti-macrophage antibody, recognizes CD163, a molecule associated with an anti-inflammatory macrophage phenotype
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DOI：
10.1369/jhc.5a6871.2006
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发表时间：
2006-07-01
影响因子：
3.2
通讯作者：
Takeya, Motohiro
中科院分区：
文献类型：
--
作者：
Komohara, Yoshihiro;Hirahara, Junko;Takeya, Motohiro
CD163是富含半胱氨酸的清道夫受体超家族的成员，仅限于单核细胞/巨噬细胞谱系，被认为是抗炎或选择性活化巨噬细胞的有用标记物。在这项研究中，我们使用质谱分析来确定抗体AM-3K识别的抗原是CD163，我们之前生成的组织巨噬细胞特异性单克隆抗体。地塞米松或白细胞介素-10刺激的抗炎亚型巨噬细胞对AM-3K表现出强烈的反应性，CD163 mRNA的表达增加。常规处理的病理标本的免疫组织化学染色显示，AM-3K识别一个特殊的巨噬细胞亚群。在肉芽肿性疾病如结核、结节病或异物反应中，肉芽肿周围的组织巨噬细胞，而肉芽肿的组成细胞如上皮样细胞和多核巨细胞，AM-3K染色阳性。在动脉粥样硬化病变中，弥漫性内膜病变中的分散巨噬细胞AM-3K呈强阳性，而动脉粥样硬化斑块中的泡沫巨噬细胞AM-3K仅呈弱染色。因此，我们认为，在常规病理标本中，AM-3K是抗炎巨噬细胞的有用标记物，因为这些细胞可以与炎症或经典活化的巨噬细胞区分开来。由于AM-3K能与大鼠、豚鼠、家兔、猫、狗、山羊、猪、牛、马、猴、鲸类等不同动物的巨噬细胞亚群发生交叉反应，因此在检测各种动物的CD163中具有广泛的应用前景。
CD163 is a member of the scavenger receptor cysteine-rich superfamily restricted to the monocyte/macrophage lineage and is thought to be a useful marker for anti-inflammatory or alternatively activated macrophages. in this study we used mass spectrometric analysis to determine that the antigen recognized by the antibody AM-3K, which we previously generated as a tissue macrophage-specific monoclonal antibody, was CD163. An anti-inflammatory subtype of macrophages stimulated by dexamethasone or interleukin-10 showed strong reactivity for AM-3K and increased expression of CD163 mRNA. Immunohistochemical staining of routinely processed pathological specimens revealed that AM-3K recognized a specialized subpopulation of macrophages. In granulomatous diseases such as tuberculosis, sarcoidosis, or foreign body reactions, tissue macrophages around granulomas, but not component cells of the granulomas such as epithelioid cells and multinucleated giant cells, showed positive staining for AM-3K. In atherosclerotic lesions, scattered macrophages in diffuse intimal lesions were strongly positive for AM-3K, whereas foamy macrophages in atheromatous plaques demonstrated only weak staining. We therefore suggest that, in routine pathological specimens, AM-3K is a useful marker for anti-inflammatory macrophages because these cells can be distinguished from inflammatory or classically activated macrophages. Because AM-3K cross-reacts with macrophage subpopulations in different animal species including rats, guinea pigs, rabbits, cats, dogs, goats, pigs, bovine species, horses, monkeys, and cetaceans, it will have wide application for detection of CD163 in various animals.