Phosphorylation-related immunoreactivity and the rate of transport of neurofilaments in chronic 2,5-hexanedione intoxication
Phosphorylation-related immunoreactivity and the rate of transport of neurofilaments in chronic 2,5-hexanedione intoxication
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DOI：
10.1016/0006-8993(91)90691-n
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发表时间：
1991-01
期刊：
影响因子：
2.9
通讯作者：
D. Watson;K. Fittro;P. Hoffman;J. Griffin
中科院分区：
文献类型：
--
作者：
D. Watson;K. Fittro;P. Hoffman;J. Griffin
本文研究了2，5-己二酮慢性中毒大鼠神经丝的轴突运输和神经丝蛋白表位的磷酸化。感觉轴突L5背根神经节表现出加速运输和减少丰富的神经丝蛋白。通过定量ELISA将抗体与磷酸化神经丝表位的结合与抗体与非磷酸化表位的结合进行比较。这种神经丝磷酸化的免疫化学指数在背根、近端外周感觉轴突和腹根中减少，但在远端神经（比目鱼肌神经）中不减少。轴突切断术产生的神经丝蛋白丰度减少与己二酮相当，而免疫化学磷酸化指数没有任何变化。这些结果与近端轴突中神经丝的磷酸化状态与神经丝运输速率相关的假设一致。
Axonal transport of neurofilaments and the phosphorylation of epitopes on neurofilament proteins was studied in rats chronically intoxicated with 2,5-hexanedione. Sensory axons arising from the L5dorsal root ganglion exhibited accelerated transport and a reduced abundance of neurofilament proteins. The binding of an antibody to phosphorylated neurofilament epitopes was compared to the binding of an antibody to non-phosphorylated epitopes by quantitative ELISA. This immunochemical index of neurofilament phosphorylation was reduced in dorsal roots, proximal peripheral sensory axons and ventral roots, but not in a distal nerve (the nerve to the soleus). Axotomy produced a reduction in neurofilament protein abundance comparable to hexanedione without any change in the immunochemical phosphorulation index. These results are consistent with the hypothesis that the state of phosphorylation of neurofilaments in proximal axons is correlated with the rate of neurofilament transport.