Influence of tissue preparation on the high-frequency acoustic properties of normal kidney tissue
Influence of tissue preparation on the high-frequency acoustic properties of normal kidney tissue
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DOI：
10.1016/s0301-5629(96)00150-0
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发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Nitta, S
中科院分区：
文献类型：
--
作者：
Sasaki, H;Saijo, Y;Nitta, S
研究了不同组织制剂对正常肾组织高频声特性的影响。将8例手术切除的正常肾组织标本分为三组：(i)新鲜冷冻切片，（ii）福尔马林固定冷冻切片，（iii）福尔马林固定石蜡切片。使用100-200 MHz频率范围内的扫描声学显微镜显示衰减常数和声速的二维分布。我们的研究结果表明：(i)三组组织之间的声学参数没有显著变化，（ii） 10%福尔马林固定对声学参数没有显著变化，（iii）在无脂肪组织区域，声学参数与制备方法无关，（iv）必须使用冷冻切片来评估富含脂肪组织的声学参数。版权所有(C) 1996年世界超声医学和生物学联合会。
The influence of various tissue preparations on the acoustic properties of normal kidney tissue at high frequencies was investigated. Eight surgically excised normal kidney tissue specimens were classified into three groups: (i) fresh, frozen section, (ii) formalin-fixed, frozen section and (iii) formalin-fixed, paraffin section. Scanning acoustic microscopy operating in the frequency range of 100-200 MHz was used to display the two-dimensional distribution of attenuation constant and sound speed. Our results indicate that (i) there is no significant variation in both acoustic parameters between the three tissue groups, (ii) fixation by 10% formalin produces no significant change in the acoustic parameters, (iii) in fat-free tissue regions, the acoustic parameters are independent of preparation method and (iv) frozen sections must be used to assess the acoustic parameters in fat-rich tissues. Copyright (C) 1996 World Federation for Ultrasound in Medicine & Biology.