Asymmetric Bioreduction of Alkenes Using Ene-Reductases YersER and KYE1 and Effects of Organic Solvents
Asymmetric Bioreduction of Alkenes Using Ene-Reductases YersER and KYE1 and Effects of Organic Solvents
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DOI：
10.1021/ol200394p
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发表时间：
2011-05-20
期刊：
影响因子：
5.2
通讯作者：
Bommarius, Andreas S.
中科院分区：
文献类型：
--
作者：
Yanto, Yanto;Winkler, Christoph K.;Bommarius, Andreas S.
不对称：来自Kluyveromycesleas的KYE 1和来自Yersiniabercovierl的Yers-ER（来自Old Yellow酶家族的两种烯还原酶）对活化烯烃的反式生物还原显示出具有中等至优异程度的立体选择性的宽底物谱。底物和酶为基础的立体控制，观察到提供相反的立体异构体的产品。有机溶剂对酶活性和立体选择性的影响进行了概述，在这项研究中，两相系统己烷和甲苯被证明，以维持生物还原效率，即使在高有机溶剂含量。
Asymmetric: trans-bioreduction of activated alkenes by KYE1 from Kluyveromyces leas and Yers-ER from Yersinia bercovierl, two ene-reductases from the Old Yellow Enzyme family, showed a broad substrate spectrum with a moderate to excellent degree of stereoselectivity. Both substrate- and enzyme-based stereocontrols were observed to furnish opposite stereoisomeric products. The effects of organic solvents on enzyme activity and stereoselectivity were outlined in this study, where two-phase systems hexane and toluene are shown to sustain bioreduction efficiency even at high organic solvent content.