Water and temperature relations of growth and ochratoxin A production by Aspergillus carbonarius strains from grapes in Europe and Israel
Water and temperature relations of growth and ochratoxin A production by Aspergillus carbonarius strains from grapes in Europe and Israel
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DOI：
10.1111/j.1365-2672.2004.02321.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Magan, N
中科院分区：
文献类型：
--
作者：
Mitchell, D;Parra, R;Magan, N
目的：研究水分活度(a(W)；0.85-0.987)和温度(10-40℃)对从欧洲三个不同国家和以色列分离的两株酿酒葡萄炭疽曲霉在以葡萄汁为代表的合成葡萄汁培养基(共8株)上生长和产赭曲霉毒素A(OTA)的影响。方法和结果：在合成葡萄汁培养基中添加甘油或葡萄糖，进行了长达56天的生长和25天的OTA产生实验。对生长前的滞后期、生长速率和赭曲霉毒素产量进行了量化。对各影响因素进行了统计比较和多元回归分析，得到了8个菌株的a(W)x温度的表面响应曲线和碳链霉菌的最佳生长和OTA产量。在边际温度和水分可利用性下，滞后期从20天增加到20天。一般说来，大多数碳曲霉菌株在30-35℃时生长最好，无论用来修饰a(W)的溶质是什么，在30-35℃时不生长。
Aims: This study investigated the in vitro effects of water activity (a(w); 0.85-0.987) and temperature (10-40degreesC) on growth and ochratoxin A (OTA) production by two strains of Aspergillus carbonarius isolated from wine grapes from three different European countries and Israel on a synthetic grape juice medium representative of mid-veraison (total of eight strains).Methods and Results: The synthetic grape juice medium was modified with glycerol or glucose and experiments carried out for up to 56 days for growth and 25 days for OTA production. The lag phase prior to growth, growth rates and ochratoxin production were quantified. Statistical comparisons were made of all factors and multiple regression analysis used to obtain surface response curves of a(w) x temperature for the eight strains and optimum growth and OTA production by A. carbonarius. The lag phase increased from 20 days at marginal temperatures and water availabilities. Generally, most A. carbonarius strains grew optimally at 30-35degreesC, regardless of solute used to modify a(w), with no growth at