Endophilin B1 is required for the maintenance of mitochondrial morphology.
Endophilin B1 is required for the maintenance of mitochondrial morphology.
复制标题
维持线粒体形态所必需的内菲林B1。
DOI：
10.1083/jcb.200407046
复制
发表时间：
2004-09-27
影响因子：
7.8
通讯作者：
Youle, Richard J
中科院分区：
文献类型：
--
作者：
Karbowski, Mariusz;Jeong, Seon-Yong;Youle, Richard J
我们报告了一种脂肪酰基转移酶，嗜内肽B1，是维持线粒体形态所必需的。这种蛋白的下调或缺乏nh2末端脂质修饰域的亲内肽B1的过度表达会导致线粒体分布和形态的显著改变。线粒体外膜室与基质分离，线粒体外膜形成囊泡和小管，这在亲内蛋白B1敲除细胞和截断蛋白过表达后也观察到，表明线粒体外膜动力学调节需要亲内蛋白B1。我们还表明，在线粒体网络的同步重塑过程中，嗜内肽B1易位到线粒体，这已经被描述为发生在细胞凋亡过程中。嗜内啡肽B1和Drp1的双敲低导致线粒体表型与Drp1的单敲低相同，这与Drp1在维持线粒体形态动力学中作用于嗜内啡肽B1上游的结果一致。
We report that a fatty acyl transferase, endophilin B1, is required for maintenance of mitochondrial morphology. Down-regulation of this protein or overexpression of endophilin B1 lacking the NH2-terminal lipid-modifying domain causes striking alterations of the mitochondrial distribution and morphology. Dissociation of the outer mitochondrial membrane compartment from that of the matrix, and formation of vesicles and tubules of outer mitochondrial membrane, was also observed in both endophilin B1 knockdown cells and after overexpression of the truncated protein, indicating that endophilin B1 is required for the regulation of the outer mitochondrial membrane dynamics. We also show that endophilin B1 translocates to the mitochondria during the synchronous remodeling of the mitochondrial network that has been described to occur during apoptosis. Double knockdown of endophilin B1 and Drp1 leads to a mitochondrial phenotype identical to that of the Drp1 single knockdown, a result consistent with Drp1 acting upstream of endophilin B1 in the maintenance of morphological dynamics of mitochondria.