Characterization of the testicular cell types present in the rat by in vivo 31P magnetic resonance spectroscopy.
Characterization of the testicular cell types present in the rat by in vivo 31P magnetic resonance spectroscopy.
复制标题
通过体内 31P 磁共振波谱表征大鼠体内睾丸细胞类型。
DOI：
10.1095/biolreprod45.1.122
复制
发表时间：
1991
影响因子：
3.6
通讯作者：
W. Mali
中科院分区：
文献类型：
--
作者：
J. van der Grond;A. V. van Pelt;C. V. van Echteld;G. Dijkstra;J. Grootegoed;D. D. de Rooij;W. Mali
用体内31P磁共振（MR）技术研究了维生素A缺乏Wistar大鼠在维生素A替代前后、子宫内辐照大鼠和对照大鼠的睾丸。睾丸磷同质酯/ATP （PM/ATP）比值从仅含间质组织和支持细胞的睾丸的0.79 +/- 0.05到以精母细胞为主要细胞类型的睾丸的1.64 +/- 0.04不等。当新一代精子进入精细胞上皮时，这一比例下降。睾丸磷酸二酯/ATP （PD/ATP）比值为0.16 +/- 0.06，以支持细胞、精原细胞或精母细胞为最先进的细胞类型。当新一代精子进入精系上皮后，PD/ATP比值迅速增加，完全发育的睾丸PD/ATP比值达到0.71 +/- 0.06。综上所述，PM/ATP比率、PD/ATP比率和pH的特定模式与精原细胞、精母细胞、圆形精母细胞和细长精母细胞的存在或生精细胞的缺失相关。因此，通过体内31P磁共振光谱可以很好地了解大鼠精系上皮的状态。
Testes of vitamin A-deficient Wistar rats before and after vitamin A replacement, of rats irradiated in utero, and of control rats were investigated by in vivo 31P magnetic resonance (MR) spectroscopy. The testicular phosphomonoester/ATP (PM/ATP) ratio ranged from 0.79 +/- 0.05 for testes that contained only interstitial tissue and Sertoli cells to 1.64 +/- 0.04 for testes in which spermatocytes were the most advanced cell types present. When new generations of spermatids entered the seminiferous epithelium, this ratio decreased. The testicular phosphodiester/ATP (PD/ATP) ratio amounted to 0.16 +/- 0.06 for testes in which Sertoli cells, spermatogonia, or spermatocytes were the most advanced cell type present. When new generations of spermatids entered the seminiferous epithelium, the PD/ATP ratio rapidly increased and finally reached a value of 0.71 +/- 0.06 for fully developed testes. Taken together, specific patterns of the PM/ATP ratio, the PD/ATP ratio, and pH were obtained that were correlated to the presence of spermatogonia, spermatocytes, round spermatids, and elongated spermatids or to the absence of spermatogenic cells. Hence, a good impression of the status of the seminiferous epithelium in the rat can be obtained by in vivo 31P MR spectroscopy.