Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique inArabidopsis thaliana
Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique inArabidopsis thaliana
复制标题
全基因组关联分析确定了调节拟南芥每粒长角果种子数的候选基因
DOI：
10.3390/plants9050585
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发表时间：
2020-05-01
期刊：
影响因子：
4.5
通讯作者：
Lin, Wen-Hui
中科院分区：
文献类型：
--
作者：
Jiang, Huan-Li;Hong, Jun;Lin, Wen-Hui
种子重量和数量最终决定种子产量。拟南芥种子数由长角果数和每角果种子数（SNS）组成。比较种子发育和重量，种子数量的决定因素在很大程度上仍然未知。在本研究中，利用 107 个可用的拟南芥种质，采用全基因组关联分析 (GWAS) 来鉴定调节 SNS 的候选基因。基于 GWAS 的基因型和表型关联分析确定了 38 个最重要的 SNP 标记位点，这些位点被映射到特定的染色体位置，使我们能够筛选数十个候选基因。基于基因表达分析，选择其中之一（PIN3）进行功能验证。它是拟南芥 SNS 的正调节因子。尽管PIN3功能缺失突变体的长角果长度没有显着变化，但其SNS和种子密度(SD)较野生型显着降低。值得注意的是，与野生型相比，由胎盘特异性启动子 STK 驱动的 PIN3 过表达系表现出明显较短的长角果、轻微降低的 SNS，但 SD 显着增加，表明无论胎盘大小如何，PIN3 通过诱导胚珠原基起始来正向调节 SD。胚珠起始决定了SNS的最大可能性，通过调节胚珠起始来调节SNS的新基因和机制值得进一步研究。
Seed weight and number ultimately determine seed yield. Arabidopsis seed number comprised of silique number and seed number per silique (SNS). Comparing seed development and weight, determinants of seed number remain largely uncharacterized. In this study, taking advantage of 107 available Arabidopsis accessions, genome-wide association analysis (GWAS) was employed to identify the candidate genes regulating SNS. GWAS-based genotype and phenotype association analysis identified 38 most significant SNPs marker sites that were mapped to specific chromosomal positions and allowed us to screen for dozens of candidate genes. One of them (PIN3) was selected for functional validation based on gene expression analysis. It is a positive regulator of Arabidopsis SNS. Although silique length ofPIN3loss of function mutant was not significantly changed, its SNS and seed density (SD) were significantly reduced as compared with the wild type. Notably,PIN3overexpression lines driven by a placenta-specific promoter STK exhibited significantly shorter siliques, slightly reduced SNS, but significant increased SD compared with wild type, suggesting thatPIN3positively regulates SD through inducing ovule primordia initiation regardless of the placenta size. Ovule initiation determines the maximal possibility of SNS, and new genes and mechanism regulating SNS through modulating ovule initiation is worth further investigated.