Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells
Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells
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DOI：
10.1016/j.immuni.2014.08.014
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发表时间：
2014-09-18
期刊：
影响因子：
32.4
通讯作者：
Brenchley, Jason M.
中科院分区：
文献类型：
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作者：
Calantone, Nina;Wu, Fan;Brenchley, Jason M.
由某些猿和人类免疫缺陷病毒（SIV和HIV）表达的病毒辅助蛋白Vpx被认为可以提高骨髓细胞的病毒感染性。我们用表达或不表达Vpx的病毒感染了35只亚洲猕猴和非洲绿色猴，并检查了体内病毒靶向细胞的情况。虽然Vpx表达的缺乏影响体内病毒动力学，但随着病毒载量的降低和CD4（+）T细胞的感染，Vpx表达对骨髓细胞的感染性没有可检测的影响。此外，病毒DNA仅在未大量耗尽CD4（+）T细胞的组织中的髓样细胞中观察到。含有病毒DNA的骨髓细胞也显示出体内T细胞吞噬的证据，表明它们的病毒DNA可能归因于SIV感染的T细胞的吞噬作用。这些数据表明，髓系细胞不是体内SIV的主要来源，与Vpx表达无关。
The viral accessory protein Vpx, expressed by certain simian and human immunodeficiency viruses (SIVs and HIVs), is thought to improve viral infectivity of myeloid cells. We infected 35 Asian macaques and African green monkeys with viruses that do or do not express Vpx and examined viral targeting of cells in vivo. While lack of Vpx expression affected viral dynamics in vivo, with decreased viral loads and infection of CD4(+) T cells, Vpx expression had no detectable effect on infectivity of myeloid cells. Moreover, viral DNA was observed only within myeloid cells in tissues not massively depleted of CD4(+) T cells. Myeloid cells containing viral DNA also showed evidence of T cell phagocytosis in vivo, suggesting that their viral DNA may be attributed to phagocytosis of SIV-infected T cells. These data suggest that myeloid cells are not a major source of SIV in vivo, irrespective of Vpx expression.