Microvesicles isolated from bovine pineal gland specifically accumulate l‐glutamate
Microvesicles isolated from bovine pineal gland specifically accumulate l‐glutamate
复制标题
从牛松果体分离的微泡特异性积累 L-谷氨酸
DOI：
10.1016/0014-5793(95)00559-r
复制
发表时间：
1995
期刊：
影响因子：
3.5
通讯作者：
A. Yamamoto
中科院分区：
文献类型：
--
作者：
Y. Moriyama;A. Yamamoto
Pinealocytes, endocrine cells that synthesize and secrete melatonin, possess a large number of synaptic‐like microvesicles (MVs) containing synaptophysin. By monitoring cross‐reactivity with anti‐synaptophysin antibody, the MVs were highly purified from bovine pineal glands. The purified MVs were morphologically similar to but distinct from neuronal synaptic vesicles by their lack of synapsin I. Immunological study indicated that the MVs contained vacuolar H+‐ATPase, synaptotagmin and synaptobrevin 2 (VAMP2). The MVs accumulatedl‐glutamate at the expense of ATP hydrolysis by vacuolar H+‐ATPase. No uptakes of melatonin, serotonin, noradrenaline, γ‐aminobutyrate or acetylcholine were observed. These results indicated that the MVs are organelles for storage ofl‐glutamate in pinealocytes and suggested a possibility that pinealocytes transmit glutamate signals by MVs‐mediated exocytosis.
DOI：
10.1083/jcb.96.5.1374
发表时间：
1983-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huttner WB;Schiebler W;Greengard P;De Camilli P
通讯作者：
De Camilli P