Benzyl butyl phthalate activates prophage, threatening the stable operation of waste activated sludge anaerobic digestion
Benzyl butyl phthalate activates prophage, threatening the stable operation of waste activated sludge anaerobic digestion
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邻苯二甲酸丁苄酯激活原噬菌体，威胁废弃活性污泥厌氧消化稳定运行
DOI：
10.1016/j.scitotenv.2020.144470
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发表时间：
2021
影响因子：
9.8
通讯作者：
Wenjun Wang
中科院分区：
文献类型：
--
作者：
Xiang Tang;Man Zhou;Changzheng Fan;Guangming Zeng;Rui Gong;Qiuxiang Xu;Biao Song;Zhaohui Yang;Yang Yang;Chengyun Zhou;Xiaoya Ren;Wenjun Wang
活性污泥厌氧消化系统的稳定运行受到大量污染物的威胁。同时，广泛的微塑料污染增加了增塑剂邻苯二甲酸苄丁酯（BBP）的环境暴露风险，WAS中BBP含量已达到相当高的水平。然而，BBP对WAS厌氧消化的影响尚不清楚。在这里，我们表明，高水平的BBP带来的厌氧消化扰乱。10.0 mg/L BBP（TSS为17，640 ± 510 mg/L的污泥）的存在导致延迟的细胞裂解，这一点通过溶解有机物和乳酸脱氢酶释放的连续平行因子分析结果得到证实。此外，证实了延迟的细胞破裂与WAS厌氧消化过程中的原噬菌体活化有关。除增溶作用外，BBP的加入还影响水解、产乙酸和产甲烷。BBP的长期影响表明，厌氧消化池中优势微生物结构稳定，但许多功能微生物的丰度发生了变化，包括短链脂肪酸生产菌和消费菌。这项工作突出了WAS厌氧消化过程的敏感性机制之一，并为新出现的污染物的环境风险的综合评估提供了新的视角。
The stable operation of the anaerobic digestion of waste activated sludge (WAS) is threatened by numerous emerging contaminants. Meanwhile, the extensive microplastic pollution increased the environmental exposure risk of plasticizer benzyl butyl phthalate (BBP), the BBP content has reached a substantial level in WAS. However, the effect of BBP on WAS anaerobic digestion is still unknown. Here we show that high-level BBP brings on anaerobic digestion upset. The presence of 10.0 mg/L BBP (in sludge with 17,640 ± 510 mg/L TSS) led to deferred cell lysis, which was confirmed by the results of continuous parallel factor analysis of dissolved organic matter and the liberation of lactate dehydrogenase. Further, the deferred cell rupture was confirmed associate with prophage activation during WAS anaerobic digestion. Besides solubilization, the hydrolysis, acetogenesis and methanogenesis were also affected by the addition of BBP. The long-term effects of BBP revealed that the dominant microbial structure in anaerobic digester was stable, but the abundance of many functional microorganisms was changed, including short chain fatty acid producers and consumers. This work highlights one of the susceptibility mechanisms for WAS anaerobic digestion processes and provides new perspectives for the comprehensive assessment of emerging contaminant's environmental risks.