Arabidopsis Calcium-Dependent Protein Kinase CPK10 Functions in Abscisic Acid- and Ca2+-Mediated Stomatal Regulation in Response to Drought Stress1[W][OA]
Arabidopsis Calcium-Dependent Protein Kinase CPK10 Functions in Abscisic Acid- and Ca2+-Mediated Stomatal Regulation in Response to Drought Stress1[W][OA]
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DOI：
10.1104/pp.110.157545
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发表时间：
2010-08
期刊：
影响因子：
7.4
通讯作者：
Jun-Jie Zou;Fengjie Wei;Cun Wang;Juan Wu;D. Ratnasekera;Wen-Xin Liu;Wei-Hua Wu
中科院分区：
文献类型：
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作者：
Jun-Jie Zou;Fengjie Wei;Cun Wang;Juan Wu;D. Ratnasekera;Wen-Xin Liu;Wei-Hua Wu
植物钙依赖性蛋白激酶（CDPKs）是一类钙离子敏感蛋白，在植物生长发育的调控以及植物对生物和非生物胁迫的响应中起着重要作用。拟南芥（Arabidopsis thaliana）基因组编码34个CDPKs，其中大部分还没有功能特征。在这里，我们报告的功能特性CPK10在拟南芥响应干旱胁迫。CPK10突变体是拟南芥CPK10基因的T-DNA插入突变体，与野生型植物相比，表现出对干旱胁迫更敏感的表型，而CPK10过表达株系表现出对干旱胁迫的耐受性增强。脱落酸（ABA）和Ca~（2+）对cpk~（10）突变体气孔关闭的诱导和气孔开放的抑制作用均受到抑制。利用酵母双杂交方法，热休克蛋白，HSP 1，被确定为CPK10相互作用蛋白。通过pull-down和双分子荧光互补实验进一步证实了CPK10和HSP 1之间的相互作用。HSP1基因敲除突变体（hsp1）在干旱胁迫下表现出与cpk10突变体相似的敏感表型，并且在气孔运动的调节中对ABA和Ca2+的敏感性降低。电生理实验表明，ABA和Ca~（2+）对气孔保卫细胞内向K~+电流的抑制作用在cpk 10和hsp 1突变体中受到削弱。所有的数据表明，CPK 10，可能通过与HSP 1相互作用，在ABA和Ca 2+介导的气孔运动的调节中发挥重要作用。
Plant calcium-dependent protein kinases (CDPKs) may function as calcium sensors and play important roles in the regulation of plant growth and development and in plant responses to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) genome encodes 34 CDPKs, and most of them have not been functionally characterized. Here, we report the functional characterization of CPK10 in Arabidopsis response to drought stress. The cpk10 mutant, a T-DNA insertion mutant for the Arabidopsis CPK10 gene, showed a much more sensitive phenotype to drought stress compared with wild-type plants, while the CPK10 overexpression lines displayed enhanced tolerance to drought stress. Induction of stomatal closure and inhibition of stomatal opening by abscisic acid (ABA) and Ca2+ were impaired in the cpk10 mutants. Using yeast two-hybrid methods, a heat shock protein, HSP1, was identified as a CPK10-interacting protein. The interaction between CPK10 and HSP1 was further confirmed by pull-down and bimolecular fluorescence complementation assays. The HSP1 knockout mutant (hsp1) plants showed a similar sensitive phenotype under drought stress as the cpk10 mutant plants and were similarly less sensitive to ABA and Ca2+ in regulation of stomatal movements. Electrophysiological experiments showed that ABA and Ca2+ inhibition of the inward K+ currents in stomatal guard cells were impaired in the cpk10 and hsp1 mutants. All presented data demonstrate that CPK10, possibly by interacting with HSP1, plays important roles in ABA- and Ca2+-mediated regulation of stomatal movements.