Engineered mutations change the structure and stability of a virus-like particle.
Engineered mutations change the structure and stability of a virus-like particle.
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DOI：
10.1021/bm300590x
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发表时间：
2012-08-13
影响因子：
6.2
通讯作者：
Finn, M. G.
中科院分区：
文献类型：
--
作者：
Fiedler, Jason D.;Higginson, Cody;Hovlid, Marisa L.;Kislukhin, Alexander A.;Castillejos, Alexandra;Manzenrieder, Florian;Campbell, Melody G.;Voss, Neil R.;Potter, Clinton S.;Carragher, Bridget;Finn, M. G.
噬菌体Qβ的单外壳蛋白(CP)自组装成T=3的二十面体病毒样颗粒(VLP)，具有广泛的应用前景。这些VLP非常稳定，但确定这种稳定性的特定分子决定因素是为了研究这些决定因素以及赋予我们的VLP材料更多功能的操作，我们操作了CP的一级结构来测试各种稳定相互作用的重要性。通过优化融合CP亚基的程序，可以很好地控制每个VLP中的平均共价二聚体数量。我们证实，二硫键是衣壳最重要的稳定元件，酸性条件显著增强了VLP对热降解的抵抗力。二聚体之间的相互作用被发现对VLP组装没有二聚体内相互作用那么重要。最后，CP的单点突变导致了三种结构形式的较小VLP的种群。
The single coat protein (CP) of bacteriophage Qβ self assembles into T=3 icosahedral virus-like particles (VLPs), of interest for a wide range of applications. These VLPs are very stable, but identification of the specific molecular determinants of this stability is To investigate these determinants along with manipulations that confer more capabilities to our VLP material, we manipulated the CP primary structure to test the importance of various stabilizing interactions. Optimization of a procedure to incorporate fused CP subunits allowed good control over the average number of covalent dimers in each VLP. We confirmed that the disulfide linkages are the most important stabilizing elements for the capsid and that acidic conditions significantly enhance the resistance of VLPs to thermal degradation. Inter-dimer interactions were found to be less important for VLP assembly than intra-dimer interactions. Finally, a single point mutation in the CP resulted in a population of smaller VLPs in three structural forms.
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影响因子：
17.1
作者：
Ashley CE;Carnes EC;Phillips GK;Durfee PN;Buley MD;Lino CA;Padilla DP;Phillips B;Carter MB;Willman CL;Brinker CJ;Caldeira Jdo C;Chackerian B;Wharton W;Peabody DS
通讯作者：
Peabody DS
影响因子：
3.8
作者：
Johnson, Harvey R.;Hooker, Jacob M.;Clark, Douglas S.
通讯作者：
Clark, Douglas S.
影响因子：
4.8
作者：
Blundell, Charles D.;Mahoney, David J.;Day, Anthony J.
通讯作者：
Day, Anthony J.
影响因子：
5.6
作者：
Lago, H;Parrott, AM;Stockley, PG
通讯作者：
Stockley, PG
影响因子：
56.9
作者：
Lee, Yun Jung;Yi, Hyunjung;Belcher, Angela M.
通讯作者：
Belcher, Angela M.