Osteoblast Isolation from Murine Calvaria and Long Bones
Osteoblast Isolation from Murine Calvaria and Long Bones
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DOI：
10.1007/978-1-61779-415-5_2
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Klein-Nulend, Jenneke
中科院分区：
文献类型：
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作者：
Bakker, Astrid D.;Klein-Nulend, Jenneke
本章描述了从成年小鼠颅骨和长骨中分离原代小鼠成骨细胞，以及从新生小鼠颅骨中分离骨细胞的过程。来自成年小鼠骨的成骨细胞作为胶原酶处理的骨片的副产物获得。新生儿颅骨成骨细胞的分离是通过骨基质的连续酶消化来实现的。由于来源和分离方法的不同，所描述的每种原代骨细胞培养物将具有其自身的特征。
This chapter describes the isolation of primary mouse osteoblasts from adult mouse calvaria and long bones, as well as the process of isolation of bone cells from neonatal mouse calvaria. Osteoblasts from adult mouse bone are obtained as outgrowth from collagenasc-treated bone pieces. Isolation of osteoblasts from neonatal calvaria is achieved by sequential enzymatic digestion of the bone matrix. Because of differences in origin and isolation method, each of the primary bone cell cultures described will have their own characteristics.