Aphidicolin: a specific inhibitor of DNA synthesis in synchronous mastocytoma P-815 cells.
Aphidicolin: a specific inhibitor of DNA synthesis in synchronous mastocytoma P-815 cells.
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Aphidicolin：同步性肥大细胞瘤 P-815 细胞 DNA 合成的特异性抑制剂。
DOI：
10.1254/jjp.30.301
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
S. Ikegami
中科院分区：
文献类型：
--
作者：
A. Ichikawa;M. Negishi;K. Tomita;S. Ikegami
以小鼠同步肥大细胞瘤P-815细胞为实验对象，研究了阿飞霉素对细胞增殖和DNA合成的影响。Aphidicolin对细胞周期S期的细胞具有特异性的细胞毒性。这种细胞毒性可以通过适当清洗药物处理的细胞来逆转，但不能通过添加脱氧核糖核苷来逆转。Aphidicolin是一种有效的DNA合成抑制剂，可选择性地抑制肥大细胞瘤细胞核和细胞质中部分纯化的DNA聚合酶α的活性，但不影响DNA聚合酶β的活性。此外，aphidicolin对RNA和蛋白质的合成没有影响，并且至少在一代内没有产生细胞大小的变化。
The influence of aphidicolin on cell multiplication and DNA synthesis was examined using synchronous mouse mastocytoma P-815 cells. Aphidicolin was cytotoxic specifically to the cells of the S phase of the cell cycle. This cytotoxicity was reversed by appropriately washing the drug-treated cells, but not by the addition of deoxyribonucleosides. Aphidicolin, a potent inhibitor of DNA synthesis, selectively inhibited the activity of partially purified DNA polymerase alpha from the nucleus and the cytosol of mastocytoma cells, but did not affect the activity of DNA polymerase beta. Furthermore, aphidicolin had no effect on the synthesis of RNA and protein, and produced no changes in cell size at least for one generation.