MxB binds to the HIV-1 core and prevents the uncoating process of HIV-1.
MxB binds to the HIV-1 core and prevents the uncoating process of HIV-1.
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DOI：
10.1186/s12977-014-0068-x
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发表时间：
2014-08-14
期刊：
影响因子：
3.3
通讯作者：
Diaz-Griffero F
中科院分区：
文献类型：
--
作者：
Fricke T;White TE;Schulte B;de Souza Aranha Vieira DA;Dharan A;Campbell EM;Brandariz-Nuñez A;Diaz-Griffero F
The IFN-α-inducible restriction factor MxB blocks HIV-1 infection after reverse transcription but prior to integration. Genetic evidence suggested that capsid is the viral determinant for restriction by MxB. This work explores the ability of MxB to bind to the HIV-1 core, and the role of capsid-binding in restriction. We showed that MxB binds to the HIV-1 core and that this interaction leads to inhibition of the uncoating process of HIV-1. These results identify MxB as an endogenously expressed protein with the ability to inhibit HIV-1 uncoating. In addition, we found that a benzimidazole-based compound known to have a binding pocket on the surface of the HIV-1 capsid prevents the binding of MxB to capsid. The use of this small-molecule identified the MxB binding region on the surface of the HIV-1 core. Domain mapping experiments revealed the following requirements for restriction: 1) MxB binding to the HIV-1 capsid, which requires the 20 N-terminal amino acids, and 2) oligomerization of MxB, which is mediated by the C-terminal domain provides the avidity for the interaction of MxB with the HIV-1 core. Overall our work establishes that MxB binds to the HIV-1 core and inhibits the uncoating process of HIV-1. Moreover, we demonstrated that HIV-1 restriction by MxB requires capsid binding and oligomerization. The online version of this article (doi:10.1186/s12977-014-0068-x) contains supplementary material, which is available to authorized users.
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影响因子：
5.3
作者：
AEBI, M;FAH, J;STAEHELI, P
通讯作者：
STAEHELI, P
影响因子：
6.7
作者：
Blair WS;Pickford C;Irving SL;Brown DG;Anderson M;Bazin R;Cao J;Ciaramella G;Isaacson J;Jackson L;Hunt R;Kjerrstrom A;Nieman JA;Patick AK;Perros M;Scott AD;Whitby K;Wu H;Butler SL
通讯作者：
Butler SL
影响因子：
3.7
作者：
Campbell, Edward M.;Perez, Omar;Hope, Thomas J.
通讯作者：
Hope, Thomas J.
影响因子：
4
作者：
Goudreau, Nathalie;Lemke, Christopher T.;Mason, Stephen W.
通讯作者：
Mason, Stephen W.
DOI：
10.1073/pnas.052430399
发表时间：
2002-03-05
影响因子：
11.1
作者：
Kochs, G;Janzen, C;Haller, O
通讯作者：
Haller, O