ENZYMATIC HETEROGENEITY OF THE CAPILLARY BED OF RAT SKELETAL-MUSCLES
ENZYMATIC HETEROGENEITY OF THE CAPILLARY BED OF RAT SKELETAL-MUSCLES
复制标题
DOI：
10.1002/aja.1001770203
复制
发表时间：
1986-10-01
影响因子：
--
通讯作者：
CARLSON, BM
中科院分区：
文献类型：
--
作者：
MRAZKOVA, O;GRIM, M;CARLSON, BM
本研究的毛细血管在大鼠骨骼肌涉及使用的组织化学方法，使一个区分动脉和静脉部分的毛细血管。在受控染色条件下，毛细血管床的动脉部分对碱性磷酸酶（AP）活性呈阳性反应，静脉部分对二肽基肽酶IV（DPP IV）活性呈阳性反应。一个短的过渡毛细管段是积极的两种酶的活性。本文对正常比目鱼肌的肌帽和胸锁乳突肌的红、白色部分进行了定量分析。定量数据表明研究的肌肉之间的毛细血管尺寸的差异。胸乳肌白色部分的毛管最长，DPP IV阳性段最短（占总毛管长度的8%）。比目鱼肌的肌帽最短，也有短的DPP IV阳性段（16%）。与此相反，DPP IV阳性的胸乳肌红部的段占总毛细血管长度的60%。调查横截面显示马赛克分布的毛细血管斑点染色AP和DPP IV活性。这项研究表明，在给定的肌纤维束内，平行于肌纤维的毛细血管以其动脉和静脉段对齐的方式相对于彼此对齐。
This study of the capillaries in rat skeletal muscle involved the use of a histochemical method that allows one to distinguish between arterial and venous portions of capillaries. Under controlled staining conditions, the arterial portion of the capillary bed reacts positively for alkaline phosphatase (AP) activity, and the venous portion is positive for dipeptidyl-peptidase IV (DPP IV) activity. A short transitional capillary segment is positive for the activity of both enzymes. Capillaries of the normal soleus muscle and the red and white portions of the sternomastoid muscle have been quantitatively analyzed. Quantitative data demonstrated differences in capillary dimensions among the muscles studied. Capillaries of the white part of the sternomastoid were the longest, and they had the shortest DPP IV-positive segment (8% of the total capillary length). Capillaries of the soleus muscle were the shortest, and they also had short DPP IV-positive segments (16%). In contrast, the DPP IV-positive segments of the red part of the sternomastoid occupied 60% of the total capillary length. Survey cross sections reveal a mosaic distribution of patches of capillaries stained for AP and DPP IV activity. This study reveals that within given bundles of muscle fibers, the capillaries that run parallel to the muscle fibers are aligned relative to one another in such a manner that their arterial and venous segments are in register.